Jiang Senping, Li Jiji, Xu Kaida, Wang Haoxue, Ye Yingying
Marine and Fisheries Institute, National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, China.
Marine and Fisheries Institute of Zhejiang Ocean University, Zhoushan, 316022, China.
Mol Biol Rep. 2025 Jul 24;52(1):750. doi: 10.1007/s11033-025-10849-w.
Due to its red blood and unique taste, Barbatia virescens is considered a species of significant economic value in Chinese coastal cities. With the ever-growing global demand for seafood, human activities have severely damaged the habitat of B. virescens, leading to the degradation of natural resources and a dramatic decline in wild population numbers.
To obtain more information on this species, genome survey sequencing was used. The genomic size of B. virescens was estimated to be 1.426 Gb, with a heterozygosity of 0.589% and a repetitive sequence content of 67.30%. Based on the sequencing data, a draft genome of 1.313 Gb was assembled, and a total of 648,158 microsatellite motifs were identified. Mononucleotide repeats were the most abundant, among which A/T and AC/GT were the dominant repeat units, accounting for 56.45% and 11.27% of the total microsatellite loci, respectively. Microsatellite loci containing 10 repeat units were the most abundant. Using 21 selected polymorphic microsatellite markers, we conducted a genetic diversity analysis of B. virescens. The number of alleles per locus ranged from 8 to 27. The mean observed heterozygosity (Ho) was 0.711, and the mean expected heterozygosity (He) was 0.874. The mean PIC value of the 21 microsatellite primers was 0.865. Thirteen loci showed significant deviations from Hardy-Weinberg equilibrium (P < 0.05).
This study comprehensively explored the genomic characteristics of B. virescens and developed 21 pairs of microsatellite primers, providing valuable data to support future research on its genetic diversity and germplasm conservation of B. virescens.
由于其红色血液和独特的味道，青蚶在中国沿海城市被认为是具有重要经济价值的物种。随着全球对海鲜需求的不断增长，人类活动严重破坏了青蚶的栖息地，导致自然资源退化和野生种群数量急剧下降。
为了获取更多关于该物种的信息，采用了基因组调查测序。估计青蚶的基因组大小为1.426 Gb，杂合度为0.589%，重复序列含量为67.30%。基于测序数据，组装了一个1.313 Gb的基因组草图，共鉴定出648,158个微卫星基序。单核苷酸重复最为丰富，其中A/T和AC/GT是主要的重复单元，分别占微卫星位点总数的56.45%和11.27%。含有10个重复单元的微卫星位点最为丰富。使用21个选定的多态性微卫星标记，对青蚶进行了遗传多样性分析。每个位点的等位基因数范围为8至27。平均观察杂合度（Ho）为0.711，平均期望杂合度（He）为0.874。21个微卫星引物的平均PIC值为0.865。13个位点显著偏离哈迪-温伯格平衡（P < 0.05）。
本研究全面探索了青蚶的基因组特征，并开发了21对微卫星引物，为未来青蚶遗传多样性和种质资源保护研究提供了有价值的数据。
