Cerqueda-García Daniel, Osorio-Paz Ixchel, Carpinteyro-Ponce Javier, Ibarra-Laclette Enrique, Altúzar-Molina Alma, Aluja Martín
Red de Manejo Biorracional de Plagas y Vectores, Instituto de Ecología, A.C. - INECOL, Clúster Científico y Tecnológico Biomimic®, Xalapa, Veracruz, Mexico.
Biosphere Sciences and Engineering Division, Carnegie Institution for Science, Baltimore, MD, United States.
Front Insect Sci. 2025 Aug 29;5:1618382. doi: 10.3389/finsc.2025.1618382. eCollection 2025.
This study explores the transcriptional dynamics of the polyphagous Mexican Fruit Fly, , across five developmental stages, revealing distinct gene expression patterns unique to each stage. We identified 9,762 DEGs associated with the four developmental stages. During the egg stage, we identified the greatest number of differentially expressed genes exhibiting a pronounced activity of metabolic pathways, particularly the Mitogen-Associated Protein Kinase (MAPK) signaling pathway, which is essential for embryonic development and defense mechanisms. The second larval instar stage mainly focused on growth, as shown by the overexpression of the Transforming Growth Factor Beta (TGF-beta) pathway. In the third larval stage, genes are significantly enriched in cuticle structure and transmembrane transport. In the pupal stage, the importance of the TGF-beta and mTOR pathways emerged, vital for tissue homeostasis and development. The adult stage exhibited sustained expression of the FOXO pathway, enhancing stress resistance crucial for survival and reproduction. Additionally, we noted differences in odor-binding protein (OBP) expression between sexes, hinting at their potential role in mating behavior. These findings provide fundamental information about the life stages of , highlighting the importance of specific signaling pathways and OBPs, which could help improve mass rearing processes and management strategies for this notorious tephritid pest.
本研究探讨了多食性墨西哥果蝇在五个发育阶段的转录动态，揭示了每个阶段独特的基因表达模式。我们鉴定出了与四个发育阶段相关的9762个差异表达基因（DEGs）。在卵期，我们鉴定出数量最多的差异表达基因，这些基因表现出代谢途径的显著活性，特别是丝裂原活化蛋白激酶（MAPK）信号通路，该通路对胚胎发育和防御机制至关重要。第二龄幼虫期主要侧重于生长，这通过转化生长因子β（TGF-β）通路的过表达得以体现。在第三龄幼虫期，基因在表皮结构和跨膜运输方面显著富集。在蛹期，TGF-β和mTOR通路的重要性显现出来，它们对组织稳态和发育至关重要。成虫期表现出FOXO通路的持续表达，增强了对生存和繁殖至关重要的抗逆性。此外，我们注意到两性之间气味结合蛋白（OBP）表达存在差异，这暗示了它们在交配行为中的潜在作用。这些发现提供了关于墨西哥果蝇生命阶段的基础信息，突出了特定信号通路和OBP的重要性，这有助于改进这种臭名昭著的实蝇害虫的大规模饲养过程和管理策略。
