Sajadi A, Kafi M, Sharifiyazdi H, Azari M
Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Vet Res. 2025;26(1):78-82. doi: 10.22099/ijvr.2025.50736.7507.
Certain limitations are associated with all kinds of diagnostic techniques used for the identification of bovine cytological endometritis under field conditions. Leukocyte esterase (LE) tape of urinary test strips has been used to detect cytological endometritis with an acceptable level of agreement with endometrial cytology in lactating dairy cows; however, the sampling procedure of LE tape strips is still time-consuming and not advisable for use at the farm level.
The present study was designed to provide a simpler and faster LE test to improve the field applicability of LE tape strips for the detection of bovine cytological endometritis.
Endometrial samples were collected using cytobrush and modified LE tape techniques from slaughtered (n=74) and live dairy cows (n=43). The urinary strip's LE tape was affixed onto the cytobrush rod head using a hot glue adhesive. This modification created the possibility of rapid reading of the intensity of color change of LE tapes.
Considering the cytology as the reference method, the sensitivity and specificity of the LE method for slaughterhouse samples were 82% and 73%, respectively. The agreement between endometrial cytology and the modified LE technique, considering the combination of slaughterhouse and clinical samples, was found to be moderate (=0.51; P<0.0001) in the present study.
A rapid leukocyte esterase test can be used as a cow side and potential alternative to endometrial cytology; however, the efficacy of this method should further be evaluated based on the conception rates in dairy cows.
在现场条件下，用于鉴定牛细胞学子宫内膜炎的各种诊断技术都存在一定局限性。尿液试纸条的白细胞酯酶（LE）试纸已被用于检测细胞学子宫内膜炎，在泌乳奶牛中与子宫内膜细胞学的一致性水平可接受；然而，LE试纸条的采样过程仍然耗时，不适合在农场层面使用。
本研究旨在提供一种更简单、更快的LE检测方法，以提高LE试纸条在检测牛细胞学子宫内膜炎方面的现场适用性。
使用细胞刷和改良的LE试纸技术，从屠宰的奶牛（n=74）和活体奶牛（n=43）中采集子宫内膜样本。将尿液试纸的LE试纸用热胶粘贴在细胞刷杆头上。这种改良使得快速读取LE试纸颜色变化强度成为可能。
以细胞学作为参考方法，LE方法对屠宰场样本的敏感性和特异性分别为82%和73%。在本研究中，考虑到屠宰场和临床样本的组合，子宫内膜细胞学与改良的LE技术之间的一致性被发现为中等（=0.51；P<0.0001）。
快速白细胞酯酶检测可作为奶牛现场检测的一种方法，是子宫内膜细胞学的潜在替代方法；然而，该方法的有效性应根据奶牛的受孕率进一步评估。
