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一种内生菌的基因组和表型分析：在胁迫条件下豌豆中的双重生物刺激和生物防治活性

Genomic and phenotypic profiling of an endophyte: dual biostimulant and biocontrol activities in pea under stress.

作者信息

Hadian Shervin, Smith Donald L, Suproniene Skaidre

机构信息

Microbiology Laboratory, Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Kėdainiai, Lithuania.

Department of Plant Science, McGill University, Montreal, QC, Canada.

出版信息

Front Microbiol. 2025 Aug 29;16:1643204. doi: 10.3389/fmicb.2025.1643204. eCollection 2025.

DOI:10.3389/fmicb.2025.1643204
PMID:40950596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12427125/
Abstract

OBJECTIVE

To evaluate the plant growth-promoting and disease-suppressing potential of sp. AR11, an endophytic bacterium isolated from , through phenotypic assessment and whole-genome analysis in pea () under normal and -stress conditions.

MATERIALS AND METHODS

Greenhouse experiments were conducted to assess the effects of AR11 inoculation on shoot and root growth, biomass, chlorophyll content, and -induced stunting. Whole-genome sequencing was performed using the PacBio SMRT platform, followed by functional annotation to identify genes related to nutrient metabolism, secondary metabolite biosynthesis, and stress adaptation. Biosafety assessment included screening for virulence and antibiotic resistance genes.

RESULTS

AR11 inoculation significantly increased shoot and root length and biomass, while reducing -induced stunting by over 70%. Under pathogen stress, treated plants showed a 67% increase in SPAD index compared to controls. Genome analysis revealed a complete 5.49 Mb circular genome with 5,175 protein-coding genes, including those for nitrogen metabolism, phosphate solubilization, siderophore production, and antifungal secondary metabolite biosynthesis. Additional features included stress-responsive regulatory systems, CRISPR-Cas loci, and DNA methylation patterns. No high-risk virulence or acquired antibiotic resistance genes were detected.

CONCLUSION

sp. AR11 is a safe, multifunctional endophytic bacterium with strong genomic and phenotypic evidence for enhancing pea growth and suppressing -related diseases, making it a promising candidate for sustainable low-input agriculture.

摘要

目的

通过在正常和盐胁迫条件下对豌豆（）进行表型评估和全基因组分析，评估从分离出的内生细菌sp. AR11促进植物生长和抑制病害的潜力。

材料与方法

进行温室试验，以评估接种AR11对地上部和根部生长、生物量、叶绿素含量以及盐诱导矮化的影响。使用PacBio SMRT平台进行全基因组测序，随后进行功能注释，以鉴定与养分代谢、次生代谢物生物合成和胁迫适应相关的基因。生物安全性评估包括筛选毒力和抗生素抗性基因。

结果

接种AR11显著增加了地上部和根部长度以及生物量，同时使盐诱导的矮化减少了70%以上。在病原菌胁迫下，处理后的植株与对照相比，SPAD指数增加了67%。基因组分析揭示了一个完整的5.49 Mb环状基因组，含有5175个蛋白质编码基因，包括氮代谢、磷溶解、铁载体产生和抗真菌次生代谢物生物合成相关的基因。其他特征包括胁迫响应调节系统、CRISPR-Cas位点和DNA甲基化模式。未检测到高风险毒力或获得性抗生素抗性基因。

结论

sp. AR11是一种安全的多功能内生细菌，具有增强豌豆生长和抑制盐相关病害的强大基因组和表型证据，使其成为可持续低投入农业的有前途候选者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9153/12427125/707c35231828/fmicb-16-1643204-g012.jpg
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本文引用的文献

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2
Plant Growth-Promoting Effect and Complete Genomic Sequence Analysis of the Beneficial Rhizosphere sp. GD-4 Isolated from .从……分离得到的有益根际菌株GD-4的促植物生长效应及全基因组序列分析
Microorganisms. 2025 Jan 27;13(2):286. doi: 10.3390/microorganisms13020286.
3
Exploring Endophytic Bacteria from spp. and Beneficial Traits on Pea Plants.
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Plants (Basel). 2024 Jun 18;13(12):1684. doi: 10.3390/plants13121684.
4
Antifungal activity of Serratia plymuthica against the phytopathogenic fungus Alternariatenuissima.粘质沙雷氏菌对植物病原菌串珠镰孢的抗真菌活性。
Microb Pathog. 2024 Aug;193:106750. doi: 10.1016/j.micpath.2024.106750. Epub 2024 Jun 19.
5
spp. as plant growth-promoting bacteria alleviating salinity, drought, and nutrient imbalance stresses.作为促进植物生长的细菌，能缓解盐分、干旱和养分失衡胁迫。
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6
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Sci Rep. 2024 Feb 27;14(1):4791. doi: 10.1038/s41598-024-55128-z.
7
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8
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Life (Basel). 2023 Jun 19;13(6):1416. doi: 10.3390/life13061416.