Maloney P C
J Bacteriol. 1979 Oct;140(1):197-205. doi: 10.1128/jb.140.1.197-205.1979.
Membrane conductance to H+ was measured in the anaerobic bacterium Streptoccus lactis by a pulse technique employing a low driving force (0.1 pH unit; 6 mV). Over the pH range of 3.7 to 8.5, a constant value for passive H+ conductance was observed, corresponding to 0.2 mumol of H+/s per p/ unit per g, dry weight (1.6 microS/cm2 of surface area). The pH insensitivity of this low basal H+ conductance supports the idea that a circulation of protons can mediate highly efficiency engery transductions across the membranes of bacteria.
采用低驱动力(0.1个pH单位;6毫伏)的脉冲技术,在厌氧细菌乳酸链球菌中测量了膜对H⁺的电导率。在3.7至8.5的pH范围内,观察到被动H⁺电导率的恒定值,相当于每克干重每p/单位每秒0.2微摩尔H⁺(表面积为1.6微西门子/平方厘米)。这种低基础H⁺电导率对pH不敏感,支持了质子循环可以介导细菌膜高效能量转换的观点。