Li Xuejuan, Dong Xiaomei, Lin Liliang
College of Life Sciences, Shaanxi Normal University, Xi'an, China Shaanxi Normal University Xi'an China.
Zookeys. 2025 Sep 4;1251:115-130. doi: 10.3897/zookeys.1251.154178. eCollection 2025.
The mitochondrial genome (mitogenome) has been widely used to infer the phylogeny, origin and evolution of insects. Although mitogenomic data have been used to study the phylogenetic relationships of Tetrigoidea (Orthoptera), larger sample sizes were also essential to explore the detailed phylogenetic relationships of these taxa. In this study, two complete mitogenomic sequences were sequenced from (formerly and ). The mitogenome sequences were assembled, annotated and analyzed. The length of the mitogenomes was 16,147 and 16,224 bp, and the nucleotide composition was A>T>C>G, A-skew and C-skew. Large intergenic regions between trnS(ucn) and ND1 were identified with a length of 191-233 bp. Phylogenetic analyses showed that Batrachideinae formed the basal position of Tetrigoidea, followed by Tripetalocerinae. The monophyly of several subfamilies was not supported. Within Tetriginae, clustered with a clade containing , and (Metrodorinae), indicating their closer phylogenetic relationship. The divergence time results indicated that Batrachideinae diverged at 170.96 Ma and Tripetalocerinae diverged at 149.36 Ma. And the divergence time between and the closely related clade was 87.06 Ma. These results represent the available mitogenome sequences of the genus and provide a valuable data resource for reconstructing phylogenetic relationships and studying the differentiation of Tetrigoidea species.
线粒体基因组（mitogenome）已被广泛用于推断昆虫的系统发育、起源和进化。尽管线粒体基因组数据已被用于研究蚱总科（直翅目）的系统发育关系，但更大的样本量对于探索这些类群详细的系统发育关系也至关重要。在本研究中，从（原 和 ）测序获得了两条完整的线粒体基因组序列。对线粒体基因组序列进行了组装、注释和分析。线粒体基因组长度分别为16,147和16,224 bp，核苷酸组成为A>T>C>G，存在A偏斜和C偏斜。在trnS(ucn)和ND1之间鉴定出大的基因间隔区，长度为191 - 233 bp。系统发育分析表明，蟾蚱亚科形成了蚱总科的基部位置，其次是三瓣蚱亚科。几个亚科的单系性未得到支持。在蚱亚科内， 与一个包含 、 和 （麦氏蚱亚科）的分支聚类，表明它们有更近的系统发育关系。分歧时间结果表明，蟾蚱亚科在170.96 Ma分歧，三瓣蚱亚科在149.36 Ma分歧。 与密切相关的 分支之间的分歧时间为87.06 Ma。这些结果代表了 属现有的线粒体基因组序列，并为重建系统发育关系和研究蚱总科物种分化提供了有价值的数据资源。
