Zeng Zhaobin, Wang Haibin, Cai Chongming, Xiang Lin, Zhou Haohuan, Xie Ziyi, Guo Bangming
Department of Neurosurgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, People's Republic of China.
Mikrochim Acta. 2025 Sep 15;192(10):670. doi: 10.1007/s00604-025-07543-w.
Methods based on photoelectrochemical immunoassay have been utilized for the quantitative monitoring of neuron-specific antigen (NSE) with neuroblastoma patients, but most provide only low sensitivity. In this contribution, a simple and feasible photoelectrochemical immunoassay based on ZIF-8-derived double-shelled hollow ZnS nanocages (ZnS NCs) was applied to the split-type photocurrent measurement of NSE in biological fluids, accompanied by an enzyme-catalyzed system for synergistic amplification. Glucose oxidase (GOx)-labeled anti-NSE secondary antibody was utilized to form a sandwiched immunoreaction on capture antibody-coated microplate, whereas ZnS NCs with strong and stable photocurrents were employed to generate the detectable photocurrent. Subsequently, GOx catalyzed the glucose substrate to produce hydrogen peroxide, thereby causing the change in the photocurrent of double-shelled hollow ZnS nanocages. Followed by optimizing experimental conditions, the photoelectrochemical immunoassay displayed good photocurrent responses within the dynamic range 1.0-10000 pg mL at a low detection limit of 0.59 pg mL NSE. Good reproducibility and high specificity were acquired for determination of NSE standards. Such a highly favourable analytical performance could be achieved even in human serum samples at different dilutions.
基于光电化学免疫分析的方法已被用于对神经母细胞瘤患者的神经元特异性抗原（NSE）进行定量监测，但大多数方法的灵敏度较低。在本研究中，一种基于ZIF-8衍生的双壳中空硫化锌纳米笼（ZnS NCs）的简单可行的光电化学免疫分析方法被应用于生物流体中NSE的分体式光电流测量，并伴有酶催化系统进行协同放大。葡萄糖氧化酶（GOx）标记的抗NSE二抗用于在包被有捕获抗体的微孔板上形成夹心免疫反应，而具有强而稳定光电流的ZnS NCs则用于产生可检测的光电流。随后，GOx催化葡萄糖底物产生过氧化氢，从而导致双壳中空硫化锌纳米笼的光电流发生变化。通过优化实验条件，该光电化学免疫分析方法在1.0 - 10000 pg mL的动态范围内表现出良好的光电流响应，NSE的检测下限为0.59 pg mL。在测定NSE标准品时具有良好的重现性和高特异性。即使在不同稀释度的人血清样品中，也能实现如此优异的分析性能。
