Li Sheng, Agudelo Garcia Paula A, Aliferis Constantin, Becich Michael J, Calyeca Jazmin, Cosgrove Benjamin D, Elisseeff Jennifer, Farzad Negin, Fertig Elana J, Glass Carolyn, Gu Liangcai, Hu Qianjiang, Ji Zhicheng, Königshoff Melanie, LeBrasseur Nathan K, Li Dongmei, Ma Anjun, Ma Qin, Menon Vilas, Mitchell Jacob T, Mora Ana L, Nagaraj Sushma, Nelson Andrew C, Niedernhofer Laura J, Rojas Mauricio, Taha Hash Brown, Wang Jinhua, Wang Siyuan, Wu Pei-Hsun, Xie Jichun, Xu Ming, Yu Miao, Zhang Xu, Zhao Yue, Adams Peter D, Aguayo-Mazzucato Cristina, Baker Darren J, Benz Christopher, Bernlohr David A, Bueno Marta, Chen Jin, Childs Bennett G, Chuang Jeffrey H, Chung Dongjun, Dileepan Mythili, Ding Li, Dong Mingze, Duncan Francesca, Enninful Archibald, Flynn William F, Franco Ana Catarina, Furman David, Garovic Vesna, Halene Stephanie, Herman Allison B, Hertzel Ann V, Iwasaki Kanako, Jeon Hyeongseon, Kang Jeon Woong, Karmakar Shilpita, Kirkland James L, Korstanje Ron, Kummerfeld Erich, Lee Jun Hee, Liu Yang, Lu Yao, Lugo-Martinez Jose, Martini Helene, Melov Simon, Musi Nicolas, Passos João F, Peters Samuel T, Rahman Irfan, Ramasamy Ramalakshmi, Rindone Alexandra N, Robbins Paul D, Robson Paul, Rodriguez-Lopez Jhonny, Rosas Lorena, Rosenthal Nadia, Schafer Marissa J, Schilling Birgit, Schmidt Elizabeth L, Schneider Kevin, Sengupta Kaustav, Shu Jian, So Peter T C, Sun Li, Tchkonia Tamar, Teneche Marcos G, Vanegas Natalia, Wang Julia, Xie Juan, Yin Shanshan, Zhang Ke, Zhu Quan, Fan Rong
Department of Cancer Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Nat Genet. 2025 Sep 15. doi: 10.1038/s41588-025-02314-y.
Cellular senescence is a complex biological process that plays a pathophysiological role in aging and age-related diseases. The biological understanding of senescence at the cellular and tissue levels remains incomplete due to the lack of specific biomarkers as well as the relative rarity of senescent cells, their phenotypic heterogeneity and dynamic features. This Review provides a comprehensive overview of multiomic approaches for the characterization and biological understanding of cellular senescence. The technical capability and challenges of each approach are discussed, and practical guidelines are provided for selecting tools for identifying, characterizing and spatially mapping senescent cells. The importance of computational analyses in multiomics research, including senescent cell identification, signature detection and interactions of senescent cells with microenvironments, is highlighted. Moreover, tissue-specific case studies and experimental design considerations for individual organs are presented. Finally, future directions and the potential impact of multiomic approaches on the biological understanding of cellular senescence are discussed.
细胞衰老 是一个复杂的生物学过程，在衰老和与年龄相关的疾病中发挥病理生理作用。由于缺乏特异性生物标志物，以及衰老细胞相对稀少、其表型异质性和动态特征，在细胞和组织水平上对衰老的生物学理解仍不完整。本综述全面概述了用于表征细胞衰老和对其进行生物学理解的多组学方法。讨论了每种方法的技术能力和挑战，并提供了选择用于识别、表征衰老细胞并对其进行空间定位的工具的实用指南。强调了计算分析在多组学研究中的重要性，包括衰老细胞识别、特征检测以及衰老细胞与微环境的相互作用。此外，还介绍了针对各个器官的组织特异性案例研究和实验设计考虑因素。最后，讨论了多组学方法在细胞衰老生物学理解方面的未来方向和潜在影响。
