Jauri Patricia Vaz, Silva Carla, Massa Adriana Montañez
Laboratorio de Microbiología del Suelo, Instituto de Ecología y Ciencias Ambientales, Universidad de La República, Iguá 4225, 11400, Montevideo, Uruguay.
Laboratorio de Microbiología del Suelo, Instituto de Ecología y Ciencias Ambientales, Universidad de La República, Iguá 4225, 11400, Montevideo, Uruguay. montanez.massa+@gmail.com.
Folia Microbiol (Praha). 2025 Sep 16. doi: 10.1007/s12223-025-01316-x.
Biofertilizers are key tools for sustainable agriculture and soil health Maintenance, yet their specific effects on soil functions and microbiota remain unclear. In order to address this, we aimed to evaluate how a biofertilizer alters soil microbial communities, physicochemical properties, and functions after 18 months of periodical use in a kaki monoculture. We found that the biofertilizer indirectly reshaped microbial community structure-especially bacterial diversity-likely through interactions with the native microbiome. Functional changes included increased microbial biomass, nitrogen mineralization, and dehydrogenase activity, with reduced acid phosphatase activity. The composition of bacterial and fungal communities exhibited significant differences between biofertilizer-treated soils and control soils across most evaluated taxonomic levels. Biodiversity was altered with biofertilizer application in bacterial communities, while fungal communities were less affected. Microbial co-occurrence networks differed between the two soil treatments, although a few patterns were consistent among treated and control soils. A novel contribution of this work is the integration of co-occurrence network analysis with microbial functional traits, revealing that core microbial networks linked to nitrogen and phosphate cycling persist despite disturbance. These findings highlight the role of microbial biodiversity and community assembly in sustaining soil functions under biofertilizer application.
生物肥料是可持续农业和维持土壤健康的关键工具，但其对土壤功能和微生物群的具体影响仍不清楚。为了解决这一问题，我们旨在评估在单一栽培的柿树中定期使用生物肥料18个月后，生物肥料如何改变土壤微生物群落、理化性质和功能。我们发现，生物肥料可能通过与本地微生物群的相互作用间接重塑微生物群落结构，尤其是细菌多样性。功能变化包括微生物生物量增加、氮矿化和脱氢酶活性增加，而酸性磷酸酶活性降低。在大多数评估的分类水平上，生物肥料处理土壤和对照土壤之间的细菌和真菌群落组成存在显著差异。生物肥料的施用改变了细菌群落的生物多样性，而真菌群落受影响较小。两种土壤处理的微生物共现网络不同，尽管在处理土壤和对照土壤中有一些模式是一致的。这项工作的一个新贡献是将共现网络分析与微生物功能特征相结合，揭示了尽管受到干扰，但与氮和磷循环相关的核心微生物网络仍然存在。这些发现突出了微生物生物多样性和群落组装在生物肥料施用下维持土壤功能中的作用。
