Paredes Paris, Flores Liset, Bustamante Mariela, Chisti Yusuf, Asenjo Juan A, Shene Carolina
Program of Doctorate in Engineering Science with Specialization in Bioprocess, Universidad de La Frontera, Temuco, 4780000, Chile.
Department of Chemical Engineering, Center of Food Biotechnology and Bioseparations, BIOREN, and Centre of Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Temuco, 4780000, Chile.
Bioresour Bioprocess. 2025 Sep 16;12(1):98. doi: 10.1186/s40643-025-00937-x.
Thraustochytrium sp. RT2316-16 is a versatile marine microbe that produces useful compounds including docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), carotenoids and coenzyme Q. Unlike the other reported Thraustochytrium spp., RT2316-16 is psychrophilic. This report identifies RT2316-16 as a potential source of squalene and assesses squalene accumulation in the biomass under different culture conditions. Furthermore, the effects of dissolved oxygen (DO) concentration on the production of fatty acids are reported for the first time. The squalene content of the biomass depended on the composition of the culture medium and the DO concentration. The accumulation of squalene was promoted when the mass ratio of glucose (or glycerol) relative to organic nitrogen was high and the DO concentration was 20% of air saturation. The highest concentration of squalene in the medium containing glucose was 1.49 ± 0.06 g L, whereas it was 0.91 ± 0.05 g L in the medium containing glycerol. Relatively high concentrations of EPA (224 ± 12 mg L) and DHA (550 ± 25 mg L) could be obtained in glucose-containing media with a DO concentration of 20%. A fed-batch operation was found to be suitable for enhancing the concentrations of EPA and DHA. Culture conditions for enhanced production of EPA, DHA and squalene by RT2316-16 are reported. Compared to other thraustochytrids, RT2316-16 was shown to be an excellent producer of squalene.
破囊壶菌属RT2316-16是一种多功能海洋微生物，能产生包括二十二碳六烯酸（DHA）、二十碳五烯酸（EPA）、类胡萝卜素和辅酶Q在内的有用化合物。与其他已报道的破囊壶菌属不同，RT2316-16是嗜冷菌。本报告确定RT2316-16为角鲨烯的潜在来源，并评估不同培养条件下角鲨烯在生物量中的积累情况。此外，首次报道了溶解氧（DO）浓度对脂肪酸产量的影响。生物量中的角鲨烯含量取决于培养基的组成和DO浓度。当葡萄糖（或甘油）与有机氮的质量比高且DO浓度为空气饱和度的20%时，角鲨烯的积累会增加。含葡萄糖的培养基中角鲨烯的最高浓度为1.49±0.06 g/L，而含甘油的培养基中为0.91±0.05 g/L。在DO浓度为20%的含葡萄糖培养基中可获得相对较高浓度的EPA（224±12 mg/L）和DHA（550±25 mg/L）。发现补料分批操作适合提高EPA和DHA的浓度。报道了RT2316-16提高EPA、DHA和角鲨烯产量的培养条件。与其他破囊壶菌相比，RT2316-16被证明是角鲨烯的优良生产者。
