Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan.
Kyushu University Future Creators in Science Project (QFC-SP) , Fukuoka, Japan.
Appl Environ Microbiol. 2023 Nov 29;89(11):e0100123. doi: 10.1128/aem.01001-23. Epub 2023 Oct 24.
Since the global market for sterols and vitamin D are grown with a high compound annual growth rate, a sustainable source of these compounds is required to keep up with the increasing demand. Thraustochytrid is a marine oleaginous microorganism that can synthesize several sterols, which are stored as SE in lipid droplets. DGAT2C is an unconventional SE synthase specific to thraustochytrids. Although the primary structure of DGAT2C shows high similarities with that of DGAT, DGAT2C utilizes sterol as an acceptor substrate instead of diacylglycerol. In this study, we examined more detailed enzymatic properties, intracellular localization, and structure-activity relationship of DGAT2C. Furthermore, we successfully developed a method to increase sterol and provitamin D3 productivity of thraustochytrid by more than threefold in the process of elucidating the function of the DGAT2C-specific N-terminal region. Our findings could lead to sustainable sterol and vitamin D production using thraustochytrid.
由于甾醇和维生素 D 的全球市场呈高速复合年增长率增长，因此需要一种可持续的来源来满足不断增长的需求。海洋产油微生物被称为厚壳虫，它可以合成多种甾醇，这些甾醇以 SE 的形式储存在脂滴中。DGAT2C 是一种非常规的 SE 合成酶，是厚壳虫所特有的。虽然 DGAT2C 的一级结构与 DGAT 有很高的相似性，但 DGAT2C 利用甾醇作为接受底物，而不是二酰基甘油。在本研究中，我们研究了 DGAT2C 的更详细的酶学特性、细胞内定位和结构-活性关系。此外，我们在阐明 DGAT2C 特异性 N 端区域功能的过程中，成功开发了一种方法，使厚壳虫中甾醇和前维生素 D3 的产量增加了两倍以上。我们的研究结果为利用厚壳虫生产可持续的甾醇和维生素 D 开辟了道路。
