泰国一份气管抽吸标本中多药耐药菌株ABU3的基因组序列草图及完整前噬菌体特征分析

Draft genome sequence and intact prophage characterisation of multidrug-resistant isolate ABU3 from a tracheal suction specimen in Thailand.

作者信息

Yasawong Montri, Santaweesuk Parweenuch, Phatcharaharikarn Manassanan, Songngamsuk Thunwarat, Wongchitrat Prapimpun, Yordpratum Umaporn, Pasharawipas Tirasak, Pochairach Runtikan, Woradulayapinij Warunya

机构信息

Program on Environmental Toxicology, Chulabhorn Graduate Institute, Bangkok 10210, Thailand.

Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI, Bangkok 10400, Thailand.

出版信息

Data Brief. 2025 Aug 22;62:112002. doi: 10.1016/j.dib.2025.112002. eCollection 2025 Oct.

DOI:10.1016/j.dib.2025.112002

PMID:40955416

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12433474/

Abstract

Multidrug-resistant ABU3 was isolated from a tracheal suction specimen from a 76-year-old female patient in Thailand. The draft genome was sequenced using Illumina NextSeq 550. The genome comprised 95 contigs totaling 3826,273 base pairs with an N50 of 112,717 bp and GC content of 38.89. Digital DNA-DNA hybridisation with ATCC 19606 revealed 80.3 % similarity. MLST assigned the isolate to ST-164, whereas MLST of the core genome showed 99.0 % similarity to cgST-4302. Resistance gene analysis identified , variants, , and (39). Antimicrobial susceptibility testing showed resistance to carbapenems, fluoroquinolones, beta-lactam combinations, extended-spectrum cephalosporins, and penicillins, with sensitivity to aminoglycosides and folate pathway inhibitors. Two intact prophage regions (23.5 and 26.3 kb) were identified containing structural and functional components. Data are available at NCBI under BioProject PRJNA550309.

摘要

多重耐药性ABU3是从泰国一名76岁女性患者的气管抽吸标本中分离出来的。使用Illumina NextSeq 550对草图基因组进行测序。该基因组由95个重叠群组成，总共3826273个碱基对，N50为112717 bp，GC含量为38.89%。与ATCC 19606进行的数字DNA-DNA杂交显示相似度为80.3%。多位点序列分型将该分离株归为ST-164，而核心基因组的多位点序列分型显示与cgST-4302的相似度为99.0%。耐药基因分析鉴定出、变体、和（39）。抗菌药物敏感性测试显示对碳青霉烯类、氟喹诺酮类、β-内酰胺类组合、超广谱头孢菌素类和青霉素类耐药，对氨基糖苷类和叶酸途径抑制剂敏感。鉴定出两个完整的前噬菌体区域（23.5和26.3 kb），包含结构和功能成分。数据可在NCBI的BioProject PRJNA550309下获取。