Kalra Sanjay, Gilada Trupti, Dabhi Ashwin, Chamle Vijay, Dhar Deepanshi, Meer Tarique, Southall Michael, Godavarthi Ashok, Patil Amol, Malve Harshad, Barlow Ashley
Consultant and Head, Department of Endocrinology, Bharti Hospital, Karnal, Haryana; University Center for Research and Development, Chandigarh University, Sahibzada Ajit Singh Nagar, Punjab, India.
Consultant Physician, Department of Infectious Diseases, Masina Hospital and Unison Medicare and Research Centre, Mumbai, Maharashtra, India.
J Assoc Physicians India. 2025 Aug;73(8S):21-27. doi: 10.59556/japi.73.1090.
Maintaining optimal hydration and electrolyte balance is essential for immune function, as it supports cellular homeostasis and immune defense mechanisms. Electrolyte-based micronutrient drinks helps replenish essential nutrients, including zinc, selenium, and vitamin E, which play key roles in modulating immune responses, mitigating oxidative stress, and regulating inflammation. Lipopolysaccharide (LPS)-induced inflammation and oxidative stress are major contributors to immune dysfunction, necessitating interventions that enhance immune resilience.
To evaluate the immunomodulatory potential of ORSL® Immunity in mitigating inflammation, enhancing phagocytic activity, and providing antioxidant protection in LPS-stimulated peripheral blood mononuclear cells (PBMCs) Materials and methods: PBMCs were isolated from human blood and stimulated with LPS to induce an inflammatory response. The effects of ORSL® Immunity+ on the immune function of treated cells were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cytotoxicity, enzyme-linked immunosorbent assay (ELISA) for inflammatory markers [tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin 6 (IL-6), interferon gamma (IFN-γ), nitric oxide (NO)], oxygen radical absorbance capacity (ORAC) assay for antioxidant capacity, and phagocytosis assay.
ORSL® Immunity+ demonstrated no cytotoxic effects on PBMCs. The treatment significantly reduced the levels of inflammatory markers, indicating an anti-inflammatory effect. Additionally, ORSL Immunity enhanced phagocytic activity in a dose-dependent manner against suggesting improved immune response. Antioxidant capacity was also significantly increased, as evidenced by the ORAC assay, highlighting its potential in mitigating oxidative stress.
ORSL® Immunity+ exhibits promising immunomodulatory properties by reducing inflammation, enhancing phagocytosis, and improving antioxidant potential in LPS-stimulated PBMCs. These findings support a potential role in immune health alongside improving hydration status, warranting further clinical evaluation.
维持最佳水合作用和电解质平衡对免疫功能至关重要，因为它支持细胞稳态和免疫防御机制。基于电解质的微量营养素饮料有助于补充必需营养素，包括锌、硒和维生素E，这些营养素在调节免疫反应、减轻氧化应激和调节炎症中起关键作用。脂多糖（LPS）诱导的炎症和氧化应激是免疫功能障碍的主要原因，因此需要采取干预措施来增强免疫恢复力。
评估ORSL®免疫饮料在减轻LPS刺激的外周血单核细胞（PBMC）炎症、增强吞噬活性和提供抗氧化保护方面的免疫调节潜力。材料和方法：从人血中分离PBMC，并用LPS刺激以诱导炎症反应。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法检测细胞毒性，酶联免疫吸附测定（ELISA）法检测炎症标志物[肿瘤坏死因子α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素6（IL-6）、干扰素γ（IFN-γ）、一氧化氮（NO）]，氧自由基吸收能力（ORAC）法检测抗氧化能力，以及吞噬试验，评估ORSL®免疫饮料对处理后细胞免疫功能的影响。
ORSL®免疫饮料对PBMC无细胞毒性作用。该处理显著降低了炎症标志物水平，表明具有抗炎作用。此外，ORSL免疫饮料以剂量依赖方式增强吞噬活性，提示免疫反应得到改善。ORAC试验证明抗氧化能力也显著增加，突出了其减轻氧化应激的潜力。
ORSL®免疫饮料通过减轻LPS刺激的PBMC炎症、增强吞噬作用和提高抗氧化潜力，展现出有前景的免疫调节特性。这些发现支持其在免疫健康方面的潜在作用，同时改善水合状态，值得进一步临床评估。
