Padilla-Garfias Francisco, Poot-Hernández Augusto César, Araiza-Villanueva Minerva, Calahorra Martha, Sánchez Norma Silvia, Peña Antonio
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico.
Unidad de Bioinformática y Manejo de la Información, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico.
Appl Environ Microbiol. 2025 Sep 16:e0155725. doi: 10.1128/aem.01557-25.
The environmental accumulation of polycyclic aromatic hydrocarbons (PAHs), such as benzo(a)pyrene (BaP), poses significant threats to ecosystems and public health due to their persistent nature, mutagenic potential, and well-documented carcinogenicity. In this study, we investigated the ability of the extremophilic yeast to activate specialized detoxification mechanisms for BaP degradation, even under nutrient-deprived conditions. When exposed to 100 ppm BaP, eliminated over 70% of the contaminant within 3 days, while maintaining normal growth dynamics. RNA-Seq analysis revealed widespread transcriptional remodeling, with 1,179 genes upregulated and 1,031 downregulated under BaP-only conditions, and 1,067 upregulated and 977 downregulated genes during cometabolic exposure (2% glucose + 100 ppm BaP), from a total of 6,506 annotated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses highlighted the activation of xenobiotic degradation pathways, notably involving cytochrome P450 monooxygenases, epoxide hydrolases, and glutathione S-transferases, alongside an enhanced antioxidant response and finely tuned glutathione homeostasis. This work presents the transcriptomic profile of BaP detoxification in , which reveals a complex transcriptional activation of genes related to stress adaptation and detoxification. Collectively, these findings suggest that could represent a promising eukaryotic platform for bioremediation.
Polycyclic aromatic hydrocarbons (PAHs), such as benzo(a)pyrene (BaP), are long-lasting environmental pollutants with serious health and ecological implications. Although microbial degradation offers a promising strategy for remediation, most efforts have focused on bacterial and filamentous fungal systems, leaving other microbial groups comparatively unexplored. In contrast, extremotolerant yeasts remain largely overlooked despite their inherent resilience. Here, we investigated the marine yeast and discovered that it not only tolerates BaP under glucose-limited conditions but also actively degrades it. This response relies on a combination of detoxifying enzymes and antioxidant defenses, reflecting a well-orchestrated metabolic adaptation to chemical stress. Our findings underscore the untapped and promising potential of as a robust option for bioremediation using eukaryotic organisms, particularly in environments where conventional microbes may fail to survive.
多环芳烃（PAHs），如苯并[a]芘（BaP），因其持久性、诱变潜力和已充分证明的致癌性，在环境中的积累对生态系统和公众健康构成重大威胁。在本研究中，我们调查了极端嗜热酵母即使在营养缺乏条件下激活专门解毒机制以降解BaP的能力。当暴露于100 ppm BaP时，在3天内消除了超过70%的污染物，同时保持正常的生长动态。RNA测序分析揭示了广泛的转录重塑，在仅BaP条件下，共有6506个注释基因，其中1179个基因上调，1031个基因下调；在共代谢暴露（2%葡萄糖 + 100 ppm BaP）期间，1067个基因上调，977个基因下调。基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析突出了异源生物降解途径的激活，特别是涉及细胞色素P450单加氧酶、环氧化物水解酶和谷胱甘肽S-转移酶，同时增强了抗氧化反应并精细调节了谷胱甘肽稳态。这项工作展示了BaP解毒在中的转录组概况，揭示了与应激适应和解毒相关基因的复杂转录激活。总体而言，这些发现表明可能是一个有前途的生物修复真核平台。
多环芳烃（PAHs），如苯并[a]芘（BaP），是具有严重健康和生态影响的持久性环境污染物。尽管微生物降解提供了一种有前途的修复策略，但大多数努力都集中在细菌和丝状真菌系统上，其他微生物群体相对未被探索。相比之下，极端耐受酵母尽管具有内在的复原力，但在很大程度上仍被忽视。在这里，我们研究了海洋酵母，发现它不仅在葡萄糖限制条件下耐受BaP，而且还能积极降解它。这种反应依赖于解毒酶和抗氧化防御的组合，反映了对化学应激的精心编排的代谢适应。我们的发现强调了作为使用真核生物进行生物修复的强大选择的未开发且有前途的潜力，特别是在传统微生物可能无法存活的环境中。
