Tsekova Albina, Drozdova Polina, Sukhovskaya Irina, Lebedeva Daria, Lysenko Liudmila, Kantserova Nadezhda
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya St, Petrozavodsk, 185910, Russia.
Institute of Biology, Irkutsk State University, 3 Lenin St, Irkutsk, 664025, Russia.
Mol Biol Rep. 2025 Sep 17;52(1):912. doi: 10.1007/s11033-025-11041-w.
Ligula intestinalisis a parasitic tapeworm with a complex life cycle. During the procercoid stage, they parasitize copepods. Later, the cestodes develop into plerocercoids in the body cavity of plankton-feeding fish before finally maturing within the intestines of piscivorous birds. The impact of plerocercoid infection on hosts may be dramatic, including organ compression, disrupted maturation and reproduction, and alter behavior. These effects, which are mediated by neuroendocrine and immunomodulatory mechanisms, can also lead to a mass fish kill. L. intestinalis and their host fish serve as a major model system for parasitological studies due to the substantial impact of these parasites on the ecology, behavior, and physiology of the fish. However, the molecular mechanisms underlying this host-parasite relationship are not well understood, in part due to limited omics data. Here, we present de novo transcriptome assembly, proteome prediction and functional annotation of the L. intestinalis plerocercoids and various tissues from infected and uninfected breams Abramis brama from Lake Syamozero (Karelia).
A total of three transcriptomes were generated using 132 M paired-end Illumina reads from L. intestinalis plerocercoids, 170 M reads from pooled tissue samples isolated from uninfected bream individuals, and 240 M from infected breams, and were assembled de novo. Additionally, we generated and annotated the predicted proteomes of both species. The transcriptome assemblies were deposited in the NCBI database (BioProject PRJNA1222162), while the translated protein sequences and functional annotations are available in a public repository.
These data will be valuable for studying molecular adaptations of cestodes to the parasitic lifestyle, as well as host-parasite relationships.
舌状绦虫是一种具有复杂生命周期的寄生绦虫。在原尾蚴阶段，它们寄生于桡足类动物。之后，绦虫在以浮游生物为食的鱼类体腔内发育成裂头蚴，最终在食鱼鸟类的肠道内成熟。裂头蚴感染对宿主的影响可能很大，包括器官受压、成熟和繁殖受阻以及行为改变。这些由神经内分泌和免疫调节机制介导的影响还可能导致大量鱼类死亡。由于这些寄生虫对鱼类的生态、行为和生理有重大影响，舌状绦虫及其宿主鱼类成为寄生虫学研究的主要模型系统。然而，这种宿主 - 寄生虫关系的分子机制尚未得到充分了解，部分原因是组学数据有限。在此，我们展示了来自塞马湖（卡累利阿）的舌状绦虫裂头蚴以及感染和未感染的鲷鱼（Abramis brama）各种组织的从头转录组组装、蛋白质组预测和功能注释。
使用来自舌状绦虫裂头蚴的1.32亿对末端Illumina读段、从未感染鲷鱼个体分离的混合组织样本的1.7亿读段以及感染鲷鱼的2.4亿读段，共生成了三个转录组，并进行了从头组装。此外，我们生成并注释了这两个物种的预测蛋白质组。转录组组装结果存于NCBI数据库（生物项目PRJNA1222162），而翻译后的蛋白质序列和功能注释可在一个公共存储库中获取。
这些数据对于研究绦虫对寄生生活方式的分子适应性以及宿主 - 寄生虫关系将具有重要价值。
