Nieto Esteban E, Ghanem Nawras, Cammarata Robertina V, Borim Corrêa Felipe, Coppotelli Bibiana M, Chatzinotas Antonis
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), Street 50 N°227, 1900, La Plata, Argentina.
Department of Applied Microbial Ecology, Helmholtz Centre for Environmental Research - UFZ, 04318, Leipzig, Germany.
Biodegradation. 2025 Sep 18;36(5):86. doi: 10.1007/s10532-025-10181-x.
Phages are a major cause of bacterial mortality, affecting bacterial diversity and ecosystem functioning. However, the impact of phage-host interactions in contaminated environments and their role in pollutant biodegradation have largely been overlooked. We isolated and characterized a novel phage that infects the PAH-degrading bacterium Paraburkholderia caledonica Bk from a polycyclic aromatic hydrocarbon (PAH)-contaminated soil and investigated the effect of different multiplicity of infection (MOI) ratios on the degradation efficiency of phenanthrene. The phage IPK is a temperate phage with a wide pH and temperature tolerance and a burst size of 80 PFU ml⁻. The phage was classified as a member of the Caudoviricetes and is related to Pseudomonas and Burkholderia phages. However, its low intergenomic similarity indicates that it is a new species. Three auxiliary metabolic genes (AMGs) related to amino acid metabolism and to bacterial growth regulation were identified in the phage genome. The highest multiplicity of infection (MOI 10) showed a rapid recovery of the host density and greater phenanthrene degradation than MOIs ranging from 0.01 to 1. This work highlights the important role of phage-host interactions in modulating the efficiency of pollutant degradation, which could be a key for improving the establishment of inoculants in bioremediation processes.
噬菌体是导致细菌死亡的主要原因，影响着细菌多样性和生态系统功能。然而，噬菌体与宿主相互作用在受污染环境中的影响及其在污染物生物降解中的作用在很大程度上被忽视了。我们从多环芳烃（PAH）污染土壤中分离并鉴定了一种新型噬菌体，它能感染PAH降解菌喀里多尼亚副伯克霍尔德菌Bk，并研究了不同感染复数（MOI）比率对菲降解效率的影响。噬菌体IPK是一种温和噬菌体，具有广泛的pH值和温度耐受性，裂解量为80 PFU ml⁻。该噬菌体被归类为有尾噬菌体目成员，与假单胞菌属和伯克霍尔德菌属噬菌体相关。然而，其基因组间的低相似性表明它是一个新物种。在噬菌体基因组中鉴定出了三个与氨基酸代谢和细菌生长调节相关的辅助代谢基因（AMG）。最高感染复数（MOI 10）显示宿主密度快速恢复，且菲降解程度高于MOI为0.01至1的情况。这项工作突出了噬菌体 - 宿主相互作用在调节污染物降解效率中的重要作用，这可能是提高生物修复过程中接种剂定殖的关键。
