Russell L B
Basic Life Sci. 1985;36:377-96. doi: 10.1007/978-1-4613-2127-9_26.
Existing and newly proposed methods to detect the induction of heritable aneuploidy are summarized, and their favorable and unfavorable features discussed. Among the tests involving direct chromosomal examination, those involving study of pronuclear chromosomes at first cleavage are judged to be the most universally informative and reliable, provided tertiary trisomy can be ruled out. Measurement of postmidterm (fetal) death is proposed as a trisomy prescreen that can be readily combined with a dominant-lethal test. Among the genetic procedures for identifying the results of malsegregation events, direct detection of aneuploids has a number of advantages over complementation methods, in which only a fraction of the products of aneuploid gametes is detectable. Direct detection of aneuploids must, however, be restricted to sex chromosomes if postnatal animals are examined. A genetic marker system to detect autosomal trisomies in fetuses is proposed. An examination of experimental parameters that might maximize induction of malsegregation leads to the recommendation to include preleptotene among exposed germ-cell stages.
总结了现有的以及新提出的检测遗传性非整倍体诱导的方法，并讨论了它们的优缺点。在涉及直接染色体检查的测试中，那些在第一次卵裂时研究原核染色体的测试被认为是最具普遍信息性和可靠性的，前提是可以排除三级三体。提议将中期后（胎儿）死亡的测量作为三体预筛查，它可以很容易地与显性致死试验相结合。在识别错分离事件结果的遗传程序中，直接检测非整倍体比互补方法有许多优势，在互补方法中，只有一部分非整倍体配子的产物是可检测的。然而，如果检查出生后的动物，直接检测非整倍体必须仅限于性染色体。提出了一种检测胎儿常染色体三体的遗传标记系统。对可能使错分离诱导最大化的实验参数进行检查后，建议在暴露的生殖细胞阶段中包括细线前期。
