Erdrich Sharon, Gelissen Ingrid C, Toma Ryan, Vuyisich Momchilo, Harnett Joanna E
School of Pharmacy, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.
Viome Life Sciences, Seattle, Washington.
ACR Open Rheumatol. 2025 Sep;7(9):e70115. doi: 10.1002/acr2.70115.
To describe the composition of the gut microbiota of women living with fibromyalgia, compared with controls, and examine the relationship between fecal microbiota and clinical features of fibromyalgia.
New Zealand women meeting American College of Rheumatology fibromyalgia criteria and age-matched controls provided fecal samples and completed validated surveys assessing pain, disorders of gut-brain interaction (DGBI), headaches, sleep quality, oral health, cognition, anxiety, depression, diet, and quality of life. Metatranscriptomic analysis identified phyla, genera, and species in fecal samples. Correlation coefficients were calculated for each α-diversity metric against clinical variables. Welch's t-test or Wilcoxon rank test was applied for binary clinical variables. Generalized linear models analyzed associations in the fibromyalgia group, adjusting for age, body mass index, and diet. Data processing and analysis used R version 4.2.1, with the Benjamini-Hochberg formula controlling for false discovery rate at ≤0.10 to minimize type 1 error risk.
No statistically significant differences were seen in the microbial expression in fecal samples of women with fibromyalgia (n = 104) compared with controls (n = 52). Significant associations among three species from the Bacteroidetes phylum and cognitive impairment were observed. Fusobacteriota was significantly associated with Rome IV(B) DGBI, specifically epigastric pain syndrome. A strong association was observed between oral microbiota in feces and upper gastrointestinal DGBI, indicating both negative and positive correlations in this population.
No overall differences in fecal microbial expression were found in women with fibromyalgia. However, Bacteroidetes and Fusobacteriota phyla were significantly associated with cognitive impairment and epigastric pain syndrome, respectively.
描述纤维肌痛女性患者的肠道微生物群组成，并与对照组进行比较，同时研究粪便微生物群与纤维肌痛临床特征之间的关系。
符合美国风湿病学会纤维肌痛标准的新西兰女性以及年龄匹配的对照组提供粪便样本，并完成经过验证的调查，评估疼痛、肠-脑交互障碍（DGBI）、头痛、睡眠质量、口腔健康、认知、焦虑、抑郁、饮食和生活质量。宏转录组分析确定粪便样本中的门、属和种。计算每个α多样性指标与临床变量的相关系数。对二元临床变量应用韦尔奇t检验或威尔科克森秩和检验。广义线性模型分析纤维肌痛组中的关联，并对年龄、体重指数和饮食进行调整。数据处理和分析使用R 4.2.1版本，采用Benjamini-Hochberg公式将错误发现率控制在≤0.10，以将I型错误风险降至最低。
与对照组（n = 52）相比，纤维肌痛女性患者（n = 104）的粪便样本微生物表达无统计学显著差异。观察到拟杆菌门的三个物种与认知障碍之间存在显著关联。梭杆菌门与罗马IV（B）型DGBI显著相关，尤其是上腹部疼痛综合征。观察到粪便中的口腔微生物群与上消化道DGBI之间存在强烈关联，表明该人群中存在负相关和正相关。
纤维肌痛女性患者的粪便微生物表达没有总体差异。然而，拟杆菌门和梭杆菌门分别与认知障碍和上腹部疼痛综合征显著相关。
