Lim Se Hyun, Mun Daye, Ryu Sangdon, Kang Min-Geun, Kang Anna, Lee Daniel Junpyo, Seo Eunsol, Son Seon-Hui, Kim Soyeon, Jang Ki Beom, Kim Bum-Keun, Park Dong-Jun, Oh Sangnam, Kim Younghoon
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, South Korea.
Honam National Institute of Biological Resources, Mokpo, 58762, South Korea.
Curr Res Food Sci. 2025 Aug 29;11:101188. doi: 10.1016/j.crfs.2025.101188. eCollection 2025.
Milk kefir is a fermented dairy product composed of a complex consortium of microorganisms, including lactic acid bacteria (LAB), yeasts, and acetic acid bacteria. Despite growing interest in its health-promoting properties, the synergistic interactions among the diverse microbial constituents of kefir remain insufficiently characterized. This study investigated the probiotic potential of milk kefir through a multi-omics approach. Two strains, SLAM 005Y and SLAM 023B, were isolated from Korean household milk kefir. Both strains exhibited key probiotic properties, including antibacterial activity, adherence to intestinal epithelial cells, and resistance in simulated gastrointestinal conditions. Supplementation with SLAM 005Y, SLAM 023B, or their synergistic combination (SYN) significantly enhanced lifespan and immune function in . Transcriptomic analysis further revealed upregulation of genes involved in longevity and antimicrobial defense, suggesting the activation of conserved stress-response pathways. In a dextran sulfate sodium (DSS)-induced murine colitis model, SYN administration led to marked improvement in clinical outcomes. SYN also restored the expression of inflammatory and anti-inflammatory cytokines and tight junction proteins, indicating improved intestinal barrier function and immune regulation. Importantly, multi-omics analysis revealed that SYN altered both gut microbial composition and fecal metabolite profiles, supporting a mechanistic link between microbiota modulation and host health. Taken together, milk kefir strains SLAM 005Y and SLAM 023B, particularly in combination, exhibit strong probiotic properties. Our findings highlight the therapeutic potential of milk kefir-originated probiotics as a functional food for improving intestinal health.
开菲尔粒奶是一种发酵乳制品，由包括乳酸菌（LAB）、酵母和醋酸菌在内的复杂微生物群落组成。尽管人们对其促进健康的特性越来越感兴趣，但开菲尔中不同微生物成分之间的协同相互作用仍未得到充分表征。本研究通过多组学方法研究了开菲尔粒奶的益生菌潜力。从韩国家庭开菲尔粒奶中分离出两株菌株，SLAM 005Y和SLAM 023B。这两株菌株均表现出关键的益生菌特性，包括抗菌活性、对肠道上皮细胞的粘附性以及在模拟胃肠道条件下的抗性。补充SLAM 005Y、SLAM 023B或它们的协同组合（SYN）显著延长了[具体对象]的寿命并增强了免疫功能。转录组分析进一步揭示了与长寿和抗菌防御相关基因的上调，表明保守应激反应途径的激活。在葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型中，给予SYN可显著改善临床结果。SYN还恢复了炎症和抗炎细胞因子以及紧密连接蛋白的表达，表明肠道屏障功能和免疫调节得到改善。重要的是，多组学分析表明SYN改变了肠道微生物组成和粪便代谢物谱，支持了微生物群调节与宿主健康之间的机制联系。综上所述，开菲尔粒奶菌株SLAM 005Y和SLAM 023B，特别是联合使用时，表现出强大的益生菌特性。我们的研究结果突出了源自开菲尔粒奶的益生菌作为改善肠道健康的功能性食品的治疗潜力。
