Tbatou Widad, Aboulghazi Abderrazak, El Ghouizi Asmae, El-Yagoubi Karima, Soulo Najoua, Benziane Ouaritini Zineb, Lyoussi Badiaa
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life (SNAMOPEQ), Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, 30000, Morocco.
The Higher Institute of Nursing Professions and Health Techniques (ISPITS), Fez, 30000, Morocco.
Avicenna J Phytomed. 2025 Sep-Oct;15(5):1450-1465. doi: 10.22038/ajp.2024.25198.
This study aims to evaluate the chemical composition antioxidant activity, and diuretic effects of Moroccan bark ethanolic extract (PPBE).
The phytochemical composition of PPBE was assessed using HPLC-DAD. Total polyphenols and flavonoids were quantified using the Folin-Ciocalteu and aluminum trichloride methods, respectively, while mineral content was determined by plasma mass spectrometry. Antioxidant activity was assessed using the reducing power assay, total antioxidant capacity, and anti-DPPH free radical assay. For the diuretic effect, sixteen male Wistar rats were divided into four groups: control (distilled water, 10 ml/kg of BW), furosemide (10 mg/kg of BW), and PPBE (200 and 400 mg/kg of BW) groups. After 15 days, plasma and urine were collected for creatinine, potassium, and sodium analysis, along with urine output measurement. Statistical analysis employed one-way ANOVA followed by Tukey multiple comparison test.
The PPBE displayed high phenolic content and potent antioxidant properties. Besides, the PPBE phenolic screening showed nine phenolic compounds with ferulate glucoside, gallic acid, and catechin as the main compounds. The PPBE demonstrated a richness in essential minerals. Furthermore, at both doses (200 and 400 mg/kg) PPBE led to a notable elevation in urine flow, urinary sodium concentration, and creatinine clearance, without affecting plasma electrolytes. In contrast, furosemide caused a reduction in plasma potassium levels.
PPBE could serve as a bioactive component, antioxidant, or preservative in food formulation. Moreover, it exhibits a diuretic effect without altering plasma composition.
本研究旨在评估摩洛哥树皮乙醇提取物（PPBE）的化学成分、抗氧化活性和利尿作用。
采用高效液相色谱 - 二极管阵列检测器（HPLC - DAD）评估PPBE的植物化学成分。分别使用福林 - 西奥尔特法和三氯化铝法对总多酚和黄酮类化合物进行定量，同时通过等离子体质谱法测定矿物质含量。使用还原力测定法、总抗氧化能力测定法和抗二苯基苦味酰基自由基（DPPH）测定法评估抗氧化活性。对于利尿作用，将16只雄性Wistar大鼠分为四组：对照组（蒸馏水，10 ml/kg体重）、呋塞米组（10 mg/kg体重）以及PPBE组（200和400 mg/kg体重）。15天后，收集血浆和尿液用于肌酐、钾和钠的分析以及尿量测量。统计分析采用单因素方差分析，随后进行Tukey多重比较检验。
PPBE显示出高酚含量和强大的抗氧化性能。此外，PPBE的酚类筛选显示出9种酚类化合物，其中阿魏酸葡萄糖苷、没食子酸和儿茶素为主要化合物。PPBE富含必需矿物质。此外，在两个剂量（200和400 mg/kg）下，PPBE均导致尿流量、尿钠浓度和肌酐清除率显著升高，且不影响血浆电解质。相比之下，呋塞米导致血浆钾水平降低。
PPBE可作为食品配方中的生物活性成分、抗氧化剂或防腐剂。此外，它具有利尿作用且不改变血浆成分。
