Sahid Muhammad Novrizal Abdi, Mogi Masaki, Maeyama Kazutaka
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia.
Department of Pharmacology, Graduate School of Medicine, Ehime University, Toon-shi, Ehime 791-0295, Japan.
Avicenna J Phytomed. 2025 Sep-Oct;15(5):1376-1384. doi: 10.22038/ajp.2024.25199.
Traditional medicine is often used to relief pain, but its use is frequently not supported by appropriate scientific information. This study aims to investigate the histamine release suppression of (SA) and (CM).
Rat peritoneal mast cells (RPMCs) and -transfected RBL-2H3 cells were activated by 50 μg/ml of compound 48/80. Water suspension of SA or CM (0.1-30 mg/ml) was used to inhibit cell activation by compound 48/80. The level of mast cell activation was determined by measuring histamine release concentration using HPLC-fluorometry.
At a concentration of 10 mg/ml, CM resulted in 22.60±5.86% in a spontaneous histamine release from RPMCs. The net histamine release after compound 48/80 stimulation in RPMC was 67.19±1.31%. CM at 3 mg/ml suppressed histamine release to 8.45±2.53%. In -transfected RBL-2H3 cells stimulated with compound 48/80, CM at concentrations of 3 and 10 mg/mL suppressed histamine release to 22.85±0.64% and 4.20±1.60%, respectively. SA at 30 mg/ml induced a spontaneous histamine release of 56.76±4.03%, compared to 5.65±2.61% in the control group. The administration of 3 mg/ml of SA to compound 48/80-stimulated RPMCs resulted in a net histamine release of 6.12±0.46%. In -transfected RBL-2H3 cells activated by compound 48/80, the net release was 35.11±3.10%. SA at 10 mg/ml suppressed histamine release to 4.88±1.42%.
SA and CM water suspension suppressed compound 48/80-induced histamine release.
传统医学常被用于缓解疼痛，但其应用往往缺乏适当的科学依据。本研究旨在探讨[具体物质A]（SA）和[具体物质B]（CM）对组胺释放的抑制作用。
用50μg/ml的化合物48/80激活大鼠腹膜肥大细胞（RPMCs）和转染[相关基因]的RBL-2H3细胞。SA或CM的水悬浮液（0.1 - 30mg/ml）用于抑制化合物48/80引起的细胞活化。通过高效液相色谱-荧光法测量组胺释放浓度来确定肥大细胞活化水平。
在浓度为10mg/ml时，CM导致RPMCs自发组胺释放率为22.60±5.86%。化合物48/80刺激后RPMCs的组胺净释放率为67.19±1.31%。3mg/ml的CM将组胺释放抑制至8.45±2.53%。在用化合物48/80刺激的转染[相关基因]的RBL-2H3细胞中，3mg/ml和10mg/ml的CM分别将组胺释放抑制至22.85±0.64%和4.20±1.60%。30mg/ml的SA诱导自发组胺释放率为56.76±4.03%，而对照组为5.65±2.61%。向化合物48/80刺激的RPMCs中加入3mg/ml的SA导致组胺净释放率为6.12±0.46%。在由化合物48/80激活的转染[相关基因]的RBL-2H3细胞中，净释放率为35.11±3.10%。10mg/ml的SA将组胺释放抑制至4.88±1.42%。
SA和CM水悬浮液抑制化合物48/80诱导的组胺释放。
