Yang Zhiqi, Wang Yankun
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Front Microbiol. 2025 Sep 3;16:1576486. doi: 10.3389/fmicb.2025.1576486. eCollection 2025.
Plant-microbial interactions shape the plant microbiome, leading to niche differentiation in microbial communities. The dynamic variation in beneficial and phytopathogenic microbes from different niches (including the roots, stems, leaves, and rhizosphere soil (RS) of plants) is poorly understood.
High-throughput sequencing was performed to explore the shifts in microbial community composition in different niches of diseased and healthy long line peppers (LLPs, L.). Correlations between microbial community composition and agronomic performance were analyzed to speculate the presence of potential pathogens and beneficial microbes in different niches and their effects on LLPs.
The relative abundance of microbial communities in the LLP different niches was dynamic. Some microbes exhibited significantly negative effects on the LLP growth and fitness, including the genera bacterial , and fungal , , , , and . Two pepper-wilt fungi and were identified according to Koch's rule, validating the study's conclusions. The pepper disease reduced plant fresh weight by 72% and increased abundance by 2-fold, additionally, LLP plant height, concentrations of leaf chlorophyll a, fruit vitamin C and fresh weight were significantly decreased contrast to healthy plants. Certainly, potential beneficial microbes (e.g., the , , and bacteria as well as the , , , , and fungi) significantly promoted some agronomic parameters of LLPs.
These finding suggested that various pathogens might be associated with pepper disease symptoms. This study lays a foundation for isolations, identifications, experimental validation of phytopathogens and beneficial microbes.
植物与微生物的相互作用塑造了植物微生物群，导致微生物群落的生态位分化。人们对来自不同生态位（包括植物的根、茎、叶和根际土壤）的有益微生物和植物病原微生物的动态变化了解甚少。
采用高通量测序技术，探究患病和健康长尖椒不同生态位微生物群落组成的变化。分析微生物群落组成与农艺性状之间的相关性，推测不同生态位中潜在病原菌和有益微生物的存在及其对长尖椒的影响。
长尖椒不同生态位微生物群落的相对丰度是动态变化的。一些微生物对长尖椒的生长和健康表现出显著的负面影响，包括细菌属、和真菌属、、、、和。根据柯赫法则鉴定出两种青枯病菌和，验证了本研究的结论。辣椒病害使植株鲜重降低了72%，并使的丰度增加了2倍，此外，与健康植株相比，长尖椒的株高、叶片叶绿素a含量、果实维生素C含量和鲜重均显著降低。当然，潜在的有益微生物（如、和细菌以及、、、和真菌）显著促进了长尖椒的一些农艺参数。
这些发现表明，各种病原菌可能与辣椒病害症状有关。本研究为植物病原菌和有益微生物的分离、鉴定及实验验证奠定了基础。
