Mekková Simona, Sondorová Miriam, Ivančová Petra, Hudáková Natália Šurín, Maďar Marián, Kadaši Marián, Mudroň Pavol
Clinic of Ruminants, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovakia.
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 041 81, Slovakia.
Vet Res Commun. 2025 Sep 19;49(6):315. doi: 10.1007/s11259-025-10897-4.
We analyzed the occurrence and distribution of selected Treponema species in dairy cows using 335 interdigital swabs, 335 fecal samples, and 25 surface swabs from bovine digital dermatitis (BDD) lesions. In surface swabs from BDD lesions, Treponema medium (92%), Treponema pedis (88%), and Treponema brennaborense (56%) were the most frequently detected species. Interdigital swabs from BDD-positive cows revealed the presence of T. medium in 40%, T. pedis in 12%, and T. brennaborense in 4% of samples. In the healthy group, T. medium was detected in 21.3%, T. pedis in 4.8%, and T. brennaborense in 1.3% of samples. Treponema species were also detected in fecal samples from both groups, with T. brennaborense found in 28% of BDD-positive cows and 22.3% of healthy cows, while T. pedis was present at a similar rate (8%) in both groups. In fecal samples from healthy cows, the proportion of T. brennaborense was significantly higher compared to T. medium and T. pedis (p < 0.001). Similarly, analysis of interdigital swabs showed a significantly higher occurrence of T. brennaborense compared to T. medium (p < 0.001). These findings highlight distinct distribution patterns of Treponema species across different sample types and suggest their potential relevance in the diagnosis and understanding of reservoir dynamics in BDD infections.
我们使用335份趾间拭子、335份粪便样本以及25份来自牛趾间皮炎（BDD）病变部位的表面拭子，分析了奶牛中特定密螺旋体物种的发生情况和分布。在BDD病变部位的表面拭子中，最常检测到的物种是中型密螺旋体（92%）、足密螺旋体（88%）和布伦纳博密螺旋体（56%）。来自BDD阳性奶牛的趾间拭子显示，40%的样本中存在中型密螺旋体，12%存在足密螺旋体，4%存在布伦纳博密螺旋体。在健康组中，21.3%的样本检测到中型密螺旋体，4.8%检测到足密螺旋体，1.3%检测到布伦纳博密螺旋体。两组的粪便样本中也检测到了密螺旋体物种，28%的BDD阳性奶牛和22.3%的健康奶牛粪便中发现了布伦纳博密螺旋体，而两组中足密螺旋体的存在率相似（8%）。在健康奶牛的粪便样本中，布伦纳博密螺旋体的比例明显高于中型密螺旋体和足密螺旋体（p < 0.001）。同样，趾间拭子分析显示，布伦纳博密螺旋体的发生率明显高于中型密螺旋体（p < 0.001）。这些发现突出了密螺旋体物种在不同样本类型中的独特分布模式，并表明它们在BDD感染的诊断和理解宿主动态方面的潜在相关性。
