Mandlik Deepa, Patil Rutuja, Arulmozhi S, Mandlik Satish
Department of Pharmacology, Bharati Vidyapeeth (Deemed to be University), Poona College of Pharmacy, Erandwane, Pune, 411038, Maharashtra, India.
J Tradit Complement Med. 2024 Jun 12;15(5):467-481. doi: 10.1016/j.jtcme.2024.06.007. eCollection 2025 Sep.
Kerala Ayurveda Ostoact Tablet (OAT) is a traditional Ayurvedic preparation that might use for the management of joint pain, bone pain and arthritis. The goal of present research was to evaluate the activity of OAT in improving postmenopausal osteoporosis (PMO) in ovariectomized (OVX) rats.
Female rats were ovariectomized bilaterally and distributed into 6 groups (n = 8) as Sham control (SC), Ovariectomy control (OVX), OVX with OAT (50 mg/kg, p.o.), OVX with OAT (100 mg/kg, p.o.) and OVX with OAT (150 mg/kg, p.o.) and Standard group (17β-estradiol, 30 μg/kg, s.c.). SC group rats went through a sham operation procedure. The animals were treated with an oral dose of OAT (50, 100 and 150 mg/kg) for 90 days. Body weight, tail skin temperature (TST) Serum hormones, bone turnover parameters, proinflammatory cytokines, bone physical parameters, histopathological analysis (uterus, vagina, and femur) and microcomputed tomography of the femur were measured on the 90th day of treatment. A new high performance thin layer chromatography (HPTLC) process has been established for the standardization of OAT.
OVX rats treated with OAT exhibited a significant decrease in TST, improved serum hormonal and lipid profile, bone turnover markers and pro-inflammatory cytokines, bone physical properties, increased bone density, and enhanced cytological and histological alterations with significant improvement in rat's body weight. In addition, OAT administration enhanced the weakening of trabecular bone microarchitecture triggered by OVX confirmed by microcomputed tomography (micro-CT). These findings imply that OAT may help treat osteoporosis in women brought on by menopause.
喀拉拉邦阿育吠陀骨力片（OAT）是一种传统的阿育吠陀制剂，可能用于治疗关节疼痛、骨痛和关节炎。本研究的目的是评估OAT改善去卵巢（OVX）大鼠绝经后骨质疏松症（PMO）的活性。
对雌性大鼠进行双侧卵巢切除，并分为6组（n = 8），分别为假手术对照组（SC）、卵巢切除对照组（OVX）、OAT治疗组（50 mg/kg，口服）、OAT治疗组（100 mg/kg，口服）、OAT治疗组（150 mg/kg，口服）和标准组（17β-雌二醇，30 μg/kg，皮下注射）。SC组大鼠接受假手术。动物口服OAT（50、100和150 mg/kg）治疗90天。在治疗的第90天测量体重、尾皮温度（TST）、血清激素、骨转换参数、促炎细胞因子、骨物理参数、组织病理学分析（子宫、阴道和股骨）以及股骨的微型计算机断层扫描。已建立一种新的高效薄层色谱（HPTLC）方法用于OAT的标准化。
用OAT治疗的OVX大鼠TST显著降低，血清激素和脂质谱、骨转换标志物和促炎细胞因子得到改善，骨物理特性增强，骨密度增加，细胞学和组织学改变增强，大鼠体重显著增加。此外，微型计算机断层扫描（micro-CT）证实，OAT给药增强了由OVX引发的小梁骨微结构的减弱。这些发现表明，OAT可能有助于治疗绝经引起的女性骨质疏松症。
