Xu Lingxia, Tan Ting, Luo Yun
The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China.
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang, China.
Biomed Chromatogr. 2025 Nov;39(11):e70225. doi: 10.1002/bmc.70225.
The medicinal herb market frequently blends the rhizome of Polygonum bistorta L. (RPB) with the rhizome of Polygonum paleaceum Wall. (RPP) for sale. Therefore, it is crucial to investigate the chemical compositions and the cytotoxic effects on lung cancer of RPB and RPP. In this study, we utilized ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS), employing diagnostic fragment ions and neutral loss techniques, to identify the chemical compositions in RPB and RPP. Furthermore, the cytotoxicity of RPB and RPP on H1299 and A549 cells was evaluated using the cell counting kit-8 (CCK-8) assay. A total of 90 compounds were tentatively characterized in RPB and RPP, including 25 gallic acid derivatives, 46 catechin derivatives, and 19 other compounds, with seven compounds verified using authentic standards. The chemical compositions of RPB and RPP were found to be largely similar, with significant differences observed only in the content of individual components. RPB and RPP exhibit significant cytotoxicity against H1299 cells, with IC values of 163.8 and 187.7 μg/mL, respectively. However, their cytotoxicity was relatively lower against A549 cells. The results indicate that there is no significant difference in the chemical composition or lung cancer cytotoxicity between RPB and RPP.
药材市场常将拳参根茎（RPB）与绵毛酸模叶蓼根茎（RPP）混合销售。因此，研究RPB和RPP的化学成分及其对肺癌的细胞毒性作用至关重要。在本研究中，我们利用超高效液相色谱-四极杆飞行时间串联质谱联用技术（UHPLC-Q-TOF-MS/MS），采用诊断性碎片离子和中性丢失技术，鉴定RPB和RPP中的化学成分。此外，使用细胞计数试剂盒-8（CCK-8）法评估RPB和RPP对H1299和A549细胞的细胞毒性。在RPB和RPP中初步鉴定出90种化合物，包括25种没食子酸衍生物、46种儿茶素衍生物和19种其他化合物，其中7种化合物通过标准品进行了验证。发现RPB和RPP的化学成分基本相似，仅个别成分含量存在显著差异。RPB和RPP对H1299细胞具有显著的细胞毒性，IC值分别为163.8和187.7μg/mL。然而，它们对A549细胞的细胞毒性相对较低。结果表明，RPB和RPP在化学成分或肺癌细胞毒性方面没有显著差异。
