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肿瘤坏死因子/核因子κB信号通路参与通络汤对盆腔炎后遗症大鼠模型作用机制的研究

TNF/NF-κB Signaling Pathway is Involved in Mechanisms of Tongluo Decoction on Rat Models with Sequelae of Pelvic Inflammatory Disease.

作者信息

Liu Baoqin, Yang Fang, Pu Xiaoqi, Yu Junjie, Wang Qing

机构信息

Traditional Chinese Medicine Gynecology, China-Japan Friendship Hospital, Beijing, People's Republic of China.

Diagnostic Radiology, China-Japan Friendship Hospital, Beijing, People's Republic of China.

出版信息

J Inflamm Res. 2025 Sep 17;18:12949-12960. doi: 10.2147/JIR.S518734. eCollection 2025.

DOI:10.2147/JIR.S518734
PMID:40984877
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12450387/
Abstract

PURPOSE

Traditional Chinese medicine can produce strong therapeutic activities for sequelae of pelvic inflammatory disease (SPID). This study aimed to investigate the efficacy and protective mechanisms of Tongluo Decoction (TLD) in the treatment of SPID.

PATIENTS AND METHODS

The damage-protective and inflammation-inhibitory effects of TLD on SPID were investigated in rat models. The possible targets of TLD against SPID were predicted using network pharmacology. The hub targets were tested in SPID rat models. Rescue experiments were conducted in human endometrial epithelial cells (HEECs). Detection of necroptosis was used the flow cytometry. ELISA was used for the quantification of inflammatory cytokines. Protein levels were detected using a Western blot assay.

RESULTS

TLD had a protective effect against uterus tissue damage caused during SPID. TLD inhibited the levels of adhesion cytokines (VEGF and TGF-β1), the tight junction proteins (ZO-1 and occludin), and inflammatory cytokines (TNF-α and IL-6). TNF, IKBKB, and NFKB1 were predicted as hub targets for TLD against SPID. TLD inhibited necroptosis of HEECs via TNF, IKBKB, and NFKB1. TLD inhibited the ratio of CD86+ M1 macrophages after differentiation induction of THP-1.

CONCLUSION

The therapeutic effects of TLD for SPID may be the result of a dual action on inflammation and necroptosis, via TNF, IKBKB, and NFKB1.

摘要

目的

中药对盆腔炎后遗症（SPID）具有强大的治疗作用。本研究旨在探讨通络汤（TLD）治疗SPID的疗效及保护机制。

患者与方法

在大鼠模型中研究TLD对SPID的损伤保护和炎症抑制作用。利用网络药理学预测TLD抗SPID的潜在靶点。在SPID大鼠模型中对核心靶点进行验证。在人子宫内膜上皮细胞（HEECs）中进行挽救实验。采用流式细胞术检测坏死性凋亡。采用酶联免疫吸附测定法（ELISA）对炎性细胞因子进行定量分析。采用蛋白质免疫印迹法检测蛋白质水平。

结果

TLD对SPID期间引起的子宫组织损伤具有保护作用。TLD抑制黏附细胞因子（VEGF和TGF-β1）、紧密连接蛋白（ZO-1和闭合蛋白）以及炎性细胞因子（TNF-α和IL-6）的水平。TNF、IKBKB和NFKB1被预测为TLD抗SPID的核心靶点。TLD通过TNF、IKBKB和NFKB1抑制HEECs的坏死性凋亡。TLD抑制THP-1分化诱导后CD86+ M1巨噬细胞的比例。

结论

TLD治疗SPID的作用机制可能是通过TNF、IKBKB和NFKB1对炎症和坏死性凋亡产生双重作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/17ce36d8d5af/JIR-18-12949-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/ee635c4d03e7/JIR-18-12949-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/eb004045c5e1/JIR-18-12949-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/1416715cf8d0/JIR-18-12949-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/405ddfffe125/JIR-18-12949-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/c8d022f8e5cf/JIR-18-12949-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/17ce36d8d5af/JIR-18-12949-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/ee635c4d03e7/JIR-18-12949-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/eb004045c5e1/JIR-18-12949-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/1416715cf8d0/JIR-18-12949-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/405ddfffe125/JIR-18-12949-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/c8d022f8e5cf/JIR-18-12949-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90fa/12450387/17ce36d8d5af/JIR-18-12949-g0006.jpg

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