Martynova Tatyana, King Bryan, Dhungana Prabin, Wei Xueyan, Kang David, Sim Cheolho
Department of Biology, Baylor University, Waco, TX 76798, USA.
USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO, United States.
Physiol Entomol. 2025 Sep;50(3):277-289. doi: 10.1111/phen.12487. Epub 2025 Mar 25.
It is imperative to determine the expression patterns and functions of genes essential for reproduction in mosquito vectors, such as , to optimize vector control strategies, such as the sterile insect technique (SIT). The reproductive organs of mosquitoes are responsible for various processes, including spermatogenesis, seminal fluid secretion, and female insemination during mating. Using Illumina RNA-seq and qRT-PCR validation, this study aimed to identify and categorize genes associated with the male accessory glands, as well as potentially other reproductive structures, of . Analysis of young and old male accessory gland tissues revealed 345 and 26 upregulated transcripts, respectively. Further qRT-PCR analysis showed that six genes were highly upregulated in old male accessory gland (MAG) tissue, including , , and . Additionally, 17 genes were found to be highly upregulated in young MAG tissue with some of them speculated to have reproductive functions; among the notably upregulated genes were those encoding , , and two . The function of (CPIJ003539) has been validated in male through in-cage insemination and fertility assays, revealing that females mated with knockdown males had impaired sperm storage and egg production abilities. These findings provide promising prospects for the development of targeted vector control strategies for mosquitoes to achieve more successful outcomes concerning efficient reduction of the pest populations.
必须确定蚊媒中生殖必需基因的表达模式和功能，例如[基因名称未给出]，以优化病媒控制策略，如昆虫不育技术（SIT）。蚊子的生殖器官负责各种过程，包括精子发生、精液分泌以及交配时的雌性交配授精。本研究利用Illumina RNA测序和qRT-PCR验证，旨在鉴定和分类与[蚊子种类未给出]雄性附腺以及潜在的其他生殖结构相关的基因。对年轻和年老雄性附腺组织的分析分别揭示了345个和26个上调的转录本。进一步的qRT-PCR分析表明，六个基因在年老雄性附腺（MAG）组织中高度上调，包括[基因名称未给出]、[基因名称未给出]和[基因名称未给出]。此外，发现17个基因在年轻MAG组织中高度上调，其中一些推测具有生殖功能；显著上调的基因中包括编码[蛋白质名称未给出]、[蛋白质名称未给出]和两个[蛋白质名称未给出]的基因。通过笼内授精和生育力测定，已在[蚊子种类未给出]雄性中验证了[基因名称CPIJ003539]的功能，结果表明与[基因名称CPIJ003539]敲低的雄性交配的雌性精子储存和产卵能力受损。这些发现为开发针对[蚊子种类未给出]蚊子的靶向病媒控制策略提供了有希望的前景，以便在有效减少害虫种群方面取得更成功的成果。
