Wilson D L
J Gen Physiol. 1971 Jan;57(1):26-40. doi: 10.1085/jgp.57.1.26.
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-(3)H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells.
将加州海兔的壁内脏神经节置于含有亮氨酸 -（3）H的培养基中孵育。从神经节中分离出单个已鉴定的神经细胞体，提取其蛋白质，并通过在5%十二烷基硫酸钠 - 聚丙烯酰胺凝胶上进行电泳分离。通过对凝胶进行染色或切片及液体闪烁计数来确定单个神经元中总蛋白质或新合成蛋白质的分布。实验表明：（a）神经细胞中大量合成了多种蛋白质；（b）不同的已鉴定神经元在凝胶中显示出可重复的不同标记模式；（c）在凝胶中显示出不同放射性分布的R2和R15细胞具有相似的染色模式；（d）大多数细胞中都有大量掺入到高分子量（>75,000）物质中。
