Zhou Yangfan, Bi Caili, Pang Tengfei, Zhuang Yanwen, Qian Yayun, Chen Fengsong
Institute of Translational Medicine, Medical College, Yangzhou University, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou 225001 P. R. China.
Department of Gastroenterology, Haimen People's Hospital Nantong 226000 People's Republic of China
RSC Adv. 2025 Sep 24;15(42):35099-35108. doi: 10.1039/d5ra05046d. eCollection 2025 Sep 22.
The accurate and sensitive detection of urease in saliva is the key to detecting () infection. In this study, we developed a pH-responsive nano-enzymatic surface-enhanced Raman scattering (SERS) sensor for the indirect detection of urease concentration, with assembled nano-enzymatic gold core-palladium shell nanorods (Au@Pd NRs) on the inner wall of the capillary. The nano-enzymatic nanorods could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide (HO) to produce oxidized TMB (ox-TMB) with a strong SERS signal. When urease is present in the liquid taken up by the capillary, it specifically hydrolyzes urea, which increases the pH of the solution, inhibits the catalytic activity of the nano-enzyme and reduces the production of ox-TMB, leading to a decrease in the SERS signal and thus enabling the indirect detection of the concentration of urease. The sensor demonstrates excellent analytical performance with a low limit of detection (LOD) of 6.09 U L, and the detection process could be completed within 15 min. For real saliva samples, the results showed significant correlation with the urease test kit, enabling rapid and accurate detection of urease concentration in saliva samples from healthy individuals and patients. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficacy of the sensor for detecting , and the analysis showed that the area under the ROC curve (AUC) was 0.959, which is promising for application in clinical diagnosis.
唾液中脲酶的准确灵敏检测是检测（）感染的关键。在本研究中，我们开发了一种用于间接检测脲酶浓度的pH响应型纳米酶表面增强拉曼散射（SERS）传感器，在毛细管内壁组装了纳米酶金核钯壳纳米棒（Au@Pd NRs）。纳米酶纳米棒可催化过氧化氢（HO）氧化3,3',5,5'-四甲基联苯胺（TMB）生成具有强SERS信号的氧化TMB（ox-TMB）。当毛细管吸取的液体中存在脲酶时，它会特异性水解尿素，使溶液pH升高，抑制纳米酶的催化活性，减少ox-TMB的产生，导致SERS信号降低，从而实现脲酶浓度的间接检测。该传感器具有出色的分析性能，检测限低至6.09 U/L，检测过程可在15分钟内完成。对于实际唾液样本，结果显示与脲酶检测试剂盒有显著相关性，能够快速准确地检测健康个体和患者唾液样本中的脲酶浓度。采用受试者工作特征（ROC）曲线评估该传感器检测（）的诊断效能，分析表明ROC曲线下面积（AUC）为0.959，在临床诊断中具有应用前景。
