循环组织蛋白酶K作为老年人肌肉减少症生物标志物的病例对照研究

Circulatory cathepsin K as biomarkers in older adults with sarcopenia: a case-control study.

作者信息

Liu Huaqing, Liu Hongdou, Xing Yan, Wang Gengze, Wang Jialin, Fan Ye, Zhang Peiwen, Wang Shangxin, Zhang Hu

机构信息

Department of Gastroenterology, Nanyang Central Hospital, Nanyang, Henan, China.

Department of Cardiovascular Surgery, Nanyang Central Hospital, Nanyang, Henan, China.

出版信息

Front Med (Lausanne). 2025 Sep 10;12:1654694. doi: 10.3389/fmed.2025.1654694. eCollection 2025.

DOI:10.3389/fmed.2025.1654694

PMID:41001380

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12457342/

Abstract

BACKGROUND

This study explores the relationship between circulating cathepsin K (CatK) and cathepsin D (CatD) levels and sarcopenia in older adults.

METHODS

This case-control study included 343 participants aged more than 65 from Nanyang Central Hospital. Sarcopenia was diagnosed using AWGS criteria, requiring low handgrip strength (HGS) and reduced appendicular skeletal muscle mass (ASM). Fasting blood samples were collected to measure CatD and CatK levels via ELISA. The study compared these levels between groups and evaluated their diagnostic value using ROC curve analysis.

RESULTS

Serum CatK levels were significantly higher in participants with low HGS, low ASM, and sarcopenia (all < 0.05). CatK negatively correlated with HGS (β = -0.899, = 0.016) and showed diagnostic value with an AUROC of 0.704 for sarcopenia. CatD levels showed no significant differences or correlations. The optimal CatK cutoff for sarcopenia was 5.53 ng/mL, with high CatK associated with increased odds of low HGS (OR = 1.895, = 0.014) and sarcopenia (OR = 3.926, < 0.001).

CONCLUSION

Circulating CatK is a promising biomarker for sarcopenia, offering potential for early diagnosis and therapeutic targeting.

摘要

背景

本研究探讨了老年人体内循环组织蛋白酶K（CatK）和组织蛋白酶D（CatD）水平与肌肉减少症之间的关系。

方法

本病例对照研究纳入了343名来自南阳中心医院的65岁以上参与者。采用亚洲肌肉减少症工作组（AWGS）标准诊断肌肉减少症，要求握力（HGS）低且四肢骨骼肌质量（ASM）降低。采集空腹血样，通过酶联免疫吸附测定（ELISA）法测量CatD和CatK水平。本研究比较了各组之间的这些水平，并使用ROC曲线分析评估了它们的诊断价值。

结果

低HGS、低ASM和肌肉减少症参与者的血清CatK水平显著更高（均P<0.05）。CatK与HGS呈负相关（β=-0.899，P=0.016），并且对肌肉减少症的诊断价值为曲线下面积（AUROC）为0.704。CatD水平无显著差异或相关性。肌肉减少症的最佳CatK临界值为5.53 ng/mL，高CatK与低HGS几率增加（比值比[OR]=1.895，P=0.014）和肌肉减少症（OR=3.926，P<0.001）相关。