Jouve Chrystèle, Rigaudiere Jean-Paul, Patrac Véronique, Capel Frédéric, Demaison Luc, Tournadre Anne
CRNH Auvergne Université Clermont Auvergne, INRAE, UMR 1019 Unité de Nutrition Humaine, 63000, Clermont-Ferrand, France.
INRAE, Unité de Nutrition Humaine, 28 Place Henri Dunant, TSA 50400, 63001, Clermont-Ferrand Cedex 1, France.
J Physiol Biochem. 2025 Sep 26. doi: 10.1007/s13105-025-01129-z.
This study aimed at determining the effects of type II collagen (CII)-induced arthritis (CIA) on cardiac homeostasis in the contexts of a laboratory chow (LC) and a Western diet (WD). The influence of dietary docosahexaenoic acid (DHA) was also examined. Sixty female Wistar rats were assigned to five groups. The first two groups were fed the LC and were treated or not with CII (LC + CIA and LC); the third and fourth groups were fed a WD with or without CII treatment (WD + CIA and WD); and the fifth group was treated with CII and it was fed the WD whose 2.5% of the lipid fraction was replaced by DHA (DHA + CIA). Ionic homeostasis, redox status, inflammation markers, and mitochondrial stress were analysed in the heart. CIA reduced the body weight and favoured wasting of the lipid and protein stores. It also reduced cardiac cell density. The CIA subgroups, particularly the WD + CIA rats, showed higher cardiac calcium and lower reduced glutathione to oxidized glutathione ratio. In the LC + CIA rats, no oxidative/nitrosative stress (ONS) was noticed and the mitochondrial extraction yield (MEY) was similar to that measured in the LC subgroup. In contrast, the ONS was higher and the MEY was lower in the WD + CIA subgroup compared to the WD one. The observed differences were not due to inflammation. DHA had little effect on the cardiac consequences of CIA. In conclusion, the WD amplified the deleterious effects of CIA on cardiac homeostasis by weakening the mitochondria via an increased ONS.
本研究旨在确定在实验室普通饲料（LC）和西方饮食（WD）背景下，II型胶原蛋白（CII）诱导的关节炎（CIA）对心脏内环境稳态的影响。同时也研究了膳食二十二碳六烯酸（DHA）的影响。将60只雌性Wistar大鼠分为五组。前两组喂食LC，分别接受或不接受CII处理（LC + CIA组和LC组）；第三组和第四组喂食WD，分别接受或不接受CII处理（WD + CIA组和WD组）；第五组接受CII处理，并喂食其脂质部分2.5%被DHA替代的WD（DHA + CIA组）。分析了心脏中的离子稳态、氧化还原状态、炎症标志物和线粒体应激。CIA降低了体重，促进了脂质和蛋白质储备的消耗。它还降低了心脏细胞密度。CIA亚组，特别是WD + CIA大鼠，心脏钙含量较高，还原型谷胱甘肽与氧化型谷胱甘肽的比值较低。在LC + CIA大鼠中，未观察到氧化/亚硝化应激（ONS），线粒体提取率（MEY）与LC亚组测得的相似。相反，与WD亚组相比，WD + CIA亚组的ONS较高，MEY较低。观察到的差异并非由炎症引起。DHA对CIA的心脏后果影响不大。总之，WD通过增加ONS削弱线粒体，放大了CIA对心脏内环境稳态的有害影响。
