Ventriglia Gianluca, Duncan Neil, Giménez Ignacio, Mylonas Constantinos C, Pousis Chrysovalentinos, Varvara Caterina, Valentini Luisa, Passantino Letizia, Corriero Aldo, Zupa Rosa
Department of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano, Italy.
Institute of Agrifood Research and Technology, 43540 La Ràpita, Spain.
Animals (Basel). 2025 Sep 11;15(18):2668. doi: 10.3390/ani15182668.
To overcome reproductive dysfunctions, recombinant gonadotropin hormones have been synthesised in different fish species. We were interested in gaining further insight into the effects of recombinant follicle-stimulating hormone (rFsh) in meagre spermatogenesis and in improving the existing knowledge on the role of testicular apoptosis. Germ cell composition and testicular apoptosis were compared in pre-pubertal fish treated with rFsh (PreP-Fsh) and controls (PreP-C), and adult meagre with testes in the proliferative (Adult-Pro) and meiotic (Adult-Meio) spermatogenesis phases. A lower density of committed spermatogonia was observed in PreP-Fsh compared with PreP-C and in Adult-Meio compared with Adult-Pro. In pre-pubertal fish and in Adult-Pro, apoptosis affected mainly individual germ cells and Sertoli cells. In Adult-Meio, a high number of cysts containing apoptotic germ cells was observed, and apoptosis often involved entire clones of germ cells. rFsh reduced testicular apoptosis in pre-pubertal fish, whereas the advancement of spermatogenesis in adults was associated with an increased density of apoptotic cells. The present study corroborated previous observations on the effects of rFsh on spermatogenesis in pre-pubertal meagre and provided further insights on the changing role of apoptosis in the pre-pubertal phase versus different phases of spermatogenesis.
为克服生殖功能障碍，已在不同鱼类中合成了重组促性腺激素。我们有兴趣进一步深入了解重组促卵泡激素（rFsh）对欧洲狼鲈精子发生的影响，并增进对睾丸细胞凋亡作用的现有认识。比较了用rFsh处理的青春期前鱼类（PreP-Fsh）和对照组（PreP-C）以及处于增殖期（Adult-Pro）和减数分裂期（Adult-Meio）精子发生阶段的成年欧洲狼鲈睾丸中的生殖细胞组成和细胞凋亡情况。与PreP-C相比，PreP-Fsh中定向精原细胞的密度较低；与Adult-Pro相比，Adult-Meio中定向精原细胞的密度也较低。在青春期前鱼类和Adult-Pro中，细胞凋亡主要影响单个生殖细胞和支持细胞。在Adult-Meio中，观察到大量含有凋亡生殖细胞的细胞囊肿，并且细胞凋亡常常涉及整个生殖细胞克隆。rFsh减少了青春期前鱼类的睾丸细胞凋亡，而成年期精子发生的进展与凋亡细胞密度增加有关。本研究证实了先前关于rFsh对青春期前欧洲狼鲈精子发生影响的观察结果，并进一步深入了解了细胞凋亡在青春期前阶段与精子发生不同阶段中不断变化的作用。
