Wang Kaiming, Li Xin, Liu Xibing, Liufu Sui, Xiao Lanlin, Chen Bohe, Chen Wenwu, Jiang Jun, Liu Yan, Ma Haiming
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Tangrenshen Group Co., Ltd., Zhuzhou 412007, China.
Animals (Basel). 2025 Sep 16;15(18):2715. doi: 10.3390/ani15182715.
Skeletal muscles, accounting for 40% of mammalian body mass, exhibit pronounced heterogeneity due to their distinct anatomical locations. Animal husbandry has focused excessively on (LDM) development while neglecting other muscles. In this study, we integrated Bulk RNA Sequencing (bulk RNA-seq) and Liquid Chromatography-Mass Spectrometry (LC-MS) analyses of (SOL), (GAS), and muscles (PMM) across three key stages in Duroc pigs. We identified nine critical genes (, , , , , , , , and ) and eight metabolites potentially involved in regulating both skeletal muscle development and fiber-type transformation. The heterogeneity between SOL and GAS was low at birth but increased gradually during development. In contrast, PMM exhibited higher heterogeneity than SOL and GAS from birth. Notably, expression levels of , , and displayed stage-specific and muscle type-dependent variations. Moreover, we observed a developmental shift from the MAPK signaling pathway (1-21 d) to the regulation of the actin cytoskeleton (21-120 d). Pairwise comparisons between the SOL, GAS, and PMM revealed that the signaling pathways were enriched in muscle fiber-type switching. Collectively, through the integration of bulk RNA-seq and LC-MS data, this study provides novel molecular breeding strategies for the genetic improvement of meat-producing animals.
骨骼肌占哺乳动物体重的40%，由于其不同的解剖位置而表现出明显的异质性。畜牧业过度关注（此处原文缺失具体肌肉名称，推测为“腰大肌”等之类的特定肌肉，用LDM代替）的发育，而忽视了其他肌肉。在本研究中，我们整合了杜洛克猪三个关键阶段的比目鱼肌（SOL）、腓肠肌（GAS）和（此处原文缺失具体肌肉名称，推测为“跖肌”等之类的特定肌肉，用PMM代替）肌肉的批量RNA测序（bulk RNA-seq）和液相色谱-质谱联用（LC-MS）分析。我们鉴定出九个关键基因（此处原文缺失具体基因名称，用一系列逗号代替）和八种可能参与调节骨骼肌发育和纤维类型转化的代谢物。出生时，SOL和GAS之间的异质性较低，但在发育过程中逐渐增加。相比之下，PMM从出生起就表现出比SOL和GAS更高的异质性。值得注意的是，（此处原文缺失具体基因名称，用一系列逗号代替）的表达水平呈现出阶段特异性和肌肉类型依赖性变化。此外，我们观察到从丝裂原活化蛋白激酶信号通路（1 - 21天）到肌动蛋白细胞骨架调节（21 - 120天）的发育转变。SOL、GAS和PMM之间的成对比较表明，信号通路在肌肉纤维类型转换中富集。总体而言，通过整合批量RNA-seq和LC-MS数据，本研究为产肉动物的遗传改良提供了新的分子育种策略。
