Perez-Silos Vanessa, Kim Hojung, Wang Chenguang, Zevallos-Morales Alejandro, Tipton Anthony, Danos-Diaz Pierina, Wilcox Ryan, Bailey Nathanael, Aggarwal Nidhi, Gisriel Savanah Dior, Smith-Hannah Alexandria, Xu Mina, Frederiksen John Karl, Murga-Zamalloa Carlos
Department of Pathology, University of Illinois Chicago, Chicago, IL 60607, USA.
Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48105, USA.
Cancers (Basel). 2025 Sep 12;17(18):2978. doi: 10.3390/cancers17182978.
Effusion-based lymphoma (EBL) is a rare and aggressive large B-cell lymphoma. It presents as a body cavity effusion without a solid mass, lacks HHV-8 association, and typically expresses CD20.
To better understand the biology of this entity, we performed transcriptomic profiling of eight EBL cases.
We analyzed the cases with the NanoString PanCancer Immune Profiling Panel and compared the results with publicly available datasets representing follicular lymphoma (FL), mantle cell lymphoma (MCL), and large B-cell lymphoma (LBCL) subtypes.
Unsupervised clustering and differential expression analysis revealed that EBL cases cluster transcriptionally with the LBCL group. Lymphoma-specific signaling pathway enrichment (SignatureDB) predominantly identified non-germinal center (activated B-cell-type) pathways. In addition, KEGG pathway analyses revealed enrichment in specific inflammatory and immune response pathways that are associated with B-cell lymphoma development in the setting of chronic inflammation, including those linked to Toll-like receptor and NF-κB signaling.
These findings support a post-germinal center origin for EBL, which arises in a background of chronic inflammation and persistent antigen stimulation.
基于积液的淋巴瘤（EBL）是一种罕见的侵袭性大B细胞淋巴瘤。它表现为体腔积液而无实体肿块，与HHV-8无关联，且通常表达CD20。
为了更好地了解该实体的生物学特性，我们对8例EBL病例进行了转录组分析。
我们使用NanoString泛癌免疫分析面板对这些病例进行分析，并将结果与代表滤泡性淋巴瘤（FL）、套细胞淋巴瘤（MCL）和大B细胞淋巴瘤（LBCL）亚型的公开可用数据集进行比较。
无监督聚类和差异表达分析显示，EBL病例在转录水平上与LBCL组聚类。淋巴瘤特异性信号通路富集（SignatureDB）主要鉴定出非生发中心（活化B细胞型）通路。此外，KEGG通路分析显示，在慢性炎症背景下与B细胞淋巴瘤发生相关的特定炎症和免疫反应通路中存在富集，包括与Toll样受体和NF-κB信号相关的通路。
这些发现支持EBL起源于生发中心后阶段，其发生于慢性炎症和持续抗原刺激的背景中。
