Todorović Petar, Maglica Mirko, Kelam Nela, Filipović Natalija, Rizikalo Azer, Perutina Ilija, Mišković Josip, Katsuyama Yu, Vukojević Katarina
Department of Anatomy, Histology and Embryology, University of Split School of Medicine, 21000 Split, Croatia.
Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina.
Life (Basel). 2025 Sep 3;15(9):1395. doi: 10.3390/life15091395.
Lung development is governed by tightly regulated signaling mechanisms, including endocytosis-mediated pathways critical for epithelial-mesenchymal communication and tissue remodeling. This study investigated the effects of deficiency on the expression of endocytic and signaling-related proteins, Megalin, Cubilin, Caveolin-1, GIPC1, and Dab2IP, during embryonic lung development in mice. Using immunofluorescence and quantitative image analysis, protein expressions were compared between and wild-type embryos at gestational days E13.5 and E15.5. Results showed significantly reduced expression of Caveolin-1 in the epithelium across both stages, along with diminished mesenchymal levels of Megalin and GIPC1 at E13.5. Cubilin and Dab2IP expression patterns showed no statistically significant differences, although developmental and compartmental shifts were observed. These findings suggest that deficiency selectively disrupts endocytic and signaling scaffolds crucial for branching morphogenesis and alveolar maturation. The altered spatiotemporal expression of these proteins underscores the essential role of in regulating lung epithelial-mesenchymal dynamics and maintaining developmental homeostasis during critical stages of organogenesis.
肺发育受严格调控的信号传导机制支配，包括对上皮-间充质通讯和组织重塑至关重要的内吞介导途径。本研究调查了[某种物质]缺乏对小鼠胚胎肺发育过程中内吞和信号相关蛋白，即巨膜蛋白（Megalin）、立方蛋白（Cubilin）、小窝蛋白-1（Caveolin-1）、GIPC1和Dab2IP表达的影响。利用免疫荧光和定量图像分析，比较了[某种物质]缺乏型和野生型胚胎在胚胎期第13.5天和第15.5天的蛋白表达情况。结果显示，在两个阶段中，[某种物质]缺乏型胚胎的上皮中小窝蛋白-1的表达均显著降低，同时在胚胎期第13.5天，间充质中巨膜蛋白和GIPC1的水平也有所下降。尽管观察到立方蛋白和Dab2IP的表达模式存在发育和区域上的变化，但无统计学显著差异。这些发现表明，[某种物质]缺乏选择性地破坏了对分支形态发生和肺泡成熟至关重要的内吞和信号支架。这些蛋白时空表达的改变强调了[某种物质]在器官发生关键阶段调节肺上皮-间充质动态和维持发育稳态中的重要作用。
