Binman Lior, Ben-Zeev Tavor, Harris Asher, Levi Chagai, Weissman Inbal, Church David D, Ferrando Arny A, Hoffman Jay R
Sport Science Program, School of Health Science, Ariel University, Ariel 40700, Israel.
Center for Translational Research in Aging and Longevity, University of Arkansas for Medical Sciences, Little Rock, AK 72205, USA.
Nutrients. 2025 Sep 16;17(18):2957. doi: 10.3390/nu17182957.
: This study examined the efficacy of essential amino acid (EAA) supplementation on changes in behavior and hippocampal neurotrophin, dopaminergic and serotonergic markers to a volume overload stress resembling an overtraining syndrome. : Thirty-two 3-month-old male C57Bl/6J mice were randomized into four groups: Resistance training (RT), resistance training with overtraining (RTO), resistance training with overtraining and EAA (RTOEAA), or control. Mice in RTOEAA received EAA supplementation (1.5 g·kg·day), while the other groups received a sham treatment. A 5-week resistance training protocol was employed. Training volume was increased two-fold during the final two weeks for RTO and RTOEAA to cause the OTS. EAA intervention for RTOEAA occurred during the OTS. : A significant decline in the maximum resistance carrying load in RTO compared to RT ( = 0.002) and RTOEAA ( = 0.029) confirmed that the animals in that group were overtrained. Significantly greater average latency times for RTO compared to RT ( = 0.009) and C ( = 0.05) indicated that the OTS caused spatial memory deficits in animals that were not supplemented. These latter changes may have been related to the significant declines in brain derived neurotrophic (BDNF) expression and elevations in dopamine 1 receptor (D1R) expressions. Increased resiliency for RTOEAA may have been related to the effect of EAA on stimulating significant increases in the expression of hippocampal tyrosine receptor kinase B (TrkB) and serotonin receptors (5-HT1A). : EAA supplementation during a resistance model of overtraining appeared to provide increased resiliency to OTS by maintaining neurotrophin expression and enhancing serotonergic adaptation.
本研究考察了补充必需氨基酸（EAA）对行为变化以及海马神经营养因子、多巴胺能和5-羟色胺能标志物的影响，该变化是针对类似于过度训练综合征的容量超负荷应激而言的。32只3月龄雄性C57Bl/6J小鼠被随机分为四组：抗阻训练（RT）组、过度训练的抗阻训练（RTO）组、过度训练且补充EAA的抗阻训练（RTOEAA）组或对照组。RTOEAA组小鼠接受EAA补充（1.5 g·kg·天），而其他组接受假处理。采用了为期5周的抗阻训练方案。在最后两周，RTO组和RTOEAA组的训练量增加了两倍以诱发过度训练综合征（OTS）。RTOEAA组的EAA干预在OTS期间进行。与RT组相比，RTO组的最大抗阻负荷显著下降（P = 0.002），与RTOEAA组相比也显著下降（P = 0.029），这证实该组动物存在过度训练。与RT组相比，RTO组的平均潜伏期显著延长（P = 0.009），与对照组相比也显著延长（P = 0.05），这表明OTS导致未补充EAA的动物出现空间记忆缺陷。这些后期变化可能与脑源性神经营养因子（BDNF）表达的显著下降以及多巴胺1受体（D1R）表达的升高有关。RTOEAA组恢复力的增强可能与EAA刺激海马酪氨酸受体激酶B（TrkB）和5-羟色胺受体（5-HT1A）表达显著增加的作用有关。在抗阻训练的过度训练模型中补充EAA似乎通过维持神经营养因子表达和增强5-羟色胺能适应性，为OTS提供了更强的恢复力。
