Manh Trieu Van, Nguyen Mai Ly Thi, Hang Ngo Thu, Giang Ngo Truong, Mao Can Van, Binh Luu Thi, Diep Nguy Thi, Sy Bui Tien, Huyen Tran Thi Thanh, Ha Vu Nhi, Cuong Le Duy, Bui Khac Cuong, Tong Hoang Van, Toan Nguyen Linh
Department of Pathophysiology, Vietnam Military Medical University, Hanoi 100000, Vietnam.
Department of Internal Medicine, Thai Nguyen University of Medicine and Pharmacy, Thai Nguyen 250000, Vietnam.
Medicina (Kaunas). 2025 Aug 28;61(9):1546. doi: 10.3390/medicina61091546.
: Rheumatoid arthritis (RA) is a systemic autoimmune inflammatory disease, and progressive arthritis is its primary clinical manifestation. The role of human parvovirus B19 (B19V) infection in the progression of RA remains unclear. This study aims to investigate the association between B19V infection and viral genetic distribution in Vietnamese RA patients. : 115 Vietnamese RA patients and 86 healthy controls (HCs) were enrolled in this observational study at the Thai Nguyen National Hospital from January 2019 to December 2021. B19V DNA was examined in serum and synovial fluid samples from RA patients using nested PCR and real-time PCR. B19V antibodies were detected in serum samples using ELISA. : B19V DNA was detected in the serum of 2 out of 115 (1.74%) RA patients but not in any HCs. Interestingly, B19V DNA was present in 12 out of 68 (17.65%) RA patients with knee effusion in their synovial fluid. Anti-B19V-IgG and anti-B19V-IgM were detected in the serum of 42.61% and 2.61% of RA patients, respectively, and in 24.42% and 12.79% of HCs, respectively. Anti-B19V-IgG levels were significantly higher in the serum of RA patients than in the serum of HCs ( = 0.007). However, anti-B19V-IgM was more commonly detected in HC serum than in RA patient serum ( = 0.006). Phylogenetic analysis showed that all B19V strains belonged to genotype 1 and subgenotype 1A. : B19V infection is frequent in RA patients and suggests a contribution of B19V to the progression of RA, particularly in a B19V genotype-1- and subgenotype-1A-dependent manner and emphasises the need for early detection and management of B19V infection in RA patients.
类风湿关节炎（RA）是一种全身性自身免疫性炎症性疾病，进行性关节炎是其主要临床表现。人细小病毒B19（B19V）感染在RA进展中的作用仍不清楚。本研究旨在调查越南RA患者中B19V感染与病毒基因分布之间的关联。2019年1月至2021年12月，在泰国谅山国立医院对115例越南RA患者和86例健康对照（HCs）进行了这项观察性研究。使用巢式PCR和实时PCR检测RA患者血清和滑液样本中的B19V DNA。使用酶联免疫吸附测定法检测血清样本中的B19V抗体。115例RA患者中有2例（1.74%）血清中检测到B19V DNA，而健康对照中均未检测到。有趣的是，68例滑液中有膝关节积液的RA患者中有12例（17.65%）存在B19V DNA。RA患者血清中抗B19V-IgG和抗B19V-IgM的检测率分别为42.61%和2.61%，HCs血清中分别为24.42%和12.79%。RA患者血清中抗B19V-IgG水平显著高于HCs血清（P = 0.007）。然而，抗B19V-IgM在HC血清中比在RA患者血清中更常见（P = 0.006）。系统发育分析表明，所有B19V毒株均属于1型基因型和1A亚型基因型。B19V感染在RA患者中很常见，提示B19V对RA进展有一定作用，特别是以B19V 1型基因型和1A亚型基因型依赖的方式，并强调需要对RA患者中的B19V感染进行早期检测和管理。
