Shi Tingting, Deng Xiaotong, Zhang Yu, Li Guohong
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming 650500, China.
Microorganisms. 2025 Aug 27;13(9):2001. doi: 10.3390/microorganisms13092001.
, a typical nematode-trapping fungus (NTF) that produces a three-dimensional adhesive network to capture nematodes, has excellent potential for the development of biocontrol agents against both plant and animal parasitic nematodes. Proteins containing the common fungal extracellular membrane (CFEM) domain are important for the nematodes' trapping by . The loss of and resulted in a significant upregulation of proteins associated with fungal pathogenicity, forming a denser adhesive material on the trap surface and ultimately increasing nematode mortality. However, the disruption of led to the opposite result. Furthermore, the deletion of not only affected trap morphology, resulting in an increased proportion of irregular traps (i.e., trap cells not fused to the hyphae), but also led to a thinner cell wall of the traps. In addition, the compensatory effects among the CFEM family and other families were demonstrated. This study revealed that the genes in participated in the formation of traps, adhesive material and cell wall, and pathogenicity, providing new insights into the functions of AfCFEM in the process of nematode trapping by NTF.
，一种典型的捕食线虫真菌（NTF），它能产生三维粘性网络来捕获线虫，在开发针对植物和动物寄生线虫的生物防治剂方面具有巨大潜力。含有常见真菌细胞外膜（CFEM）结构域的蛋白质对于线虫被 捕获至关重要。 和 的缺失导致与真菌致病性相关的蛋白质显著上调，在陷阱表面形成更致密的粘性物质，最终增加线虫死亡率。然而， 的破坏导致了相反的结果。此外， 的缺失不仅影响陷阱形态，导致不规则陷阱（即陷阱细胞未与菌丝融合）的比例增加，还导致陷阱的细胞壁变薄。此外，还证明了CFEM家族与其他家族之间的补偿作用。本研究表明， 中的 基因参与了陷阱、粘性物质和细胞壁的形成以及致病性，为AfCFEM在NTF捕获线虫过程中的功能提供了新的见解。
