Pitters Malte, Fritsch Henrik, Su Ang, Jung Klaus, Becher Paul
Institute of Virology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, D-30559 Hannover, Germany.
Institute of Animal Genomics, University of Veterinary Medicine Hannover, D-30559 Hannover, Germany.
Viruses. 2025 Aug 29;17(9):1188. doi: 10.3390/v17091188.
Bovine respiratory disease complex (BRDC) is a multifactorial and globally prevalent condition involving a combination of viral and bacterial pathogens, as well as environmental stressors. Viral agents often initiate infections in the upper respiratory tract (URT), predisposing animals to secondary bacterial infections and severe clinical manifestations. Among the key viral contributors to BRDC are bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BHV-1). In this study, submerged liquid cultures of undifferentiated bovine nasal epithelial cells (BNECs) were employed to investigate mono- and co-infections with BVDV and BHV-1. Epithelial barrier integrity was assessed to evaluate the cytopathic effects of BHV-1, while viral replication and release were quantified. Both viruses demonstrated polarized release, and BHV-1 infection exhibited a pronounced cytopathic effect. Notably, a preceding BVDV infection did not alter the progression or outcome of BHV-1 infection in this in vitro model. These findings suggest that primary BNEC cultures represent a valuable and physiologically relevant tool for studying viral dynamics and interactions associated with BRDC.
牛呼吸道疾病综合征（BRDC）是一种多因素且在全球普遍存在的病症，涉及病毒和细菌病原体以及环境应激因素的综合作用。病毒病原体通常在上呼吸道（URT）引发感染，使动物易发生继发性细菌感染和出现严重的临床表现。BRDC的主要病毒病原体包括牛病毒性腹泻病毒（BVDV）和牛疱疹病毒1型（BHV-1）。在本研究中，利用未分化牛鼻上皮细胞（BNECs）的深层液体培养来研究BVDV和BHV-1的单一感染和共同感染。评估上皮屏障完整性以评价BHV-1的细胞病变效应，同时对病毒复制和释放进行定量分析。两种病毒均表现出极性释放，且BHV-1感染表现出明显的细胞病变效应。值得注意的是，在这个体外模型中，先前的BVDV感染并未改变BHV-1感染的进程或结果。这些发现表明，原代BNEC培养物是研究与BRDC相关的病毒动态和相互作用的有价值且与生理相关的工具。
