Dos Santos Caroline Fernandes, Nunes Priscila Conrado Guerra, Fiestas-Solorzano Victor Edgar, Gandini Mariana, Dos Santos Flavia Barreto, Pinheiro Roberta Olmo, de Souza Luís Jose, Damasco Paulo Vieira, de Oliveira Pinto Luzia Maria, de Azeredo Elzinandes Leal
Laboratório das Interações Vírus Hospedeiros, Instituto Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil.
Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil.
Viruses. 2025 Sep 7;17(9):1224. doi: 10.3390/v17091224.
Chikungunya fever (CF), caused by the Chikungunya virus (CHIKV), is characterized by disabling symptoms such as joint pain that can last for months. Monocytes play a central role in immune modulation and viral replication during infection. This study evaluated the clinical and immunological profiles of patients with laboratory-confirmed CF. Fever and joint pain were the most frequently reported symptoms, whereas edema was more common in women. CHIKV-infect individuals exhibited increased TLR4 expression in non-classical monocytes (CD14+CD16++). Additionally, intermediate (CD14+CD16+) and non-classical (CD14+CD16++) monocytes expressing TLR7 were enriched during the acute phase and in some chronic patients, thereby suggest prolonged TLR7 pathway activation. Levels of soluble CD163 (sCD163)-a marker of monocyte/macrophage activation-were elevated as well, indicating sustained immune activation. Coagulation-related mediators-including Tissue factor (TF) and Tissue factor pathway inhibitor (TFPI)-also increased, despite the rarity of hemorrhagic events or thrombocytopenia. Patients with arthritis demonstrated higher frequencies of TLR7+ intermediate monocytes and elevated Epidermal growth factor (EGF) levels, whereas those with edema exhibit increased Vascular endothelial growth factor (VEGF) levels. Overall, these findings highlighted the differential activation of CD16+ monocytes and suggested that sCD163 is a marker of monocyte/macrophage activation during CHIKV infection.
基孔肯雅热（CF）由基孔肯雅病毒（CHIKV）引起，其特征为出现诸如关节疼痛等致残症状，且疼痛可持续数月。单核细胞在感染期间的免疫调节和病毒复制中起核心作用。本研究评估了实验室确诊的CF患者的临床和免疫学特征。发热和关节疼痛是最常报告的症状，而水肿在女性中更为常见。感染CHIKV的个体在非经典单核细胞（CD14+CD16++）中表现出TLR4表达增加。此外，表达TLR7的中间型（CD14+CD16+）和非经典型（CD14+CD16++）单核细胞在急性期和一些慢性患者中增多，从而提示TLR7途径的激活持续存在。可溶性CD163（sCD163）——单核细胞/巨噬细胞激活的标志物——水平也升高，表明免疫激活持续存在。尽管出血事件或血小板减少症罕见，但包括组织因子（TF）和组织因子途径抑制剂（TFPI）在内的凝血相关介质也增加。患有关节炎的患者表现出较高频率的TLR7+中间型单核细胞和升高的表皮生长因子（EGF）水平，而患有水肿的患者表现出血管内皮生长因子（VEGF）水平升高。总体而言，这些发现突出了CD16+单核细胞的差异激活，并表明sCD163是CHIKV感染期间单核细胞/巨噬细胞激活的标志物。
