来自离子载体A23187刺激的人白血病嗜碱性粒细胞的慢反应物质（SRS）。I. 花生四烯酸前体作用的证据及初步纯化。
Slow reacting substance (SRS) from ionophore A23187-stimulated human leukemic basophils. I. Evidence for a precursor role of arachidonic acid and initial purification.
作者信息
Yecies L D, Wedner H J, Parker C W
出版信息
J Immunol. 1979 Dec;123(6):2814-6.
PMID:41019
Abstract
SRS was generated from human leukemic basophils upon stimulation with ionophore A23187. Radiolabel from [14C]-AA was incorporated into SRS with continued comigration of radioactivity and bioactivity through several chromatographic systems including DEAE-cellulose, silicic acid, and RP-HPLC. Human basophilic leukemia SRS displayed physiochemical properties similar to those of rat basophilic leukemia cell SRS.
摘要
在用离子载体A23187刺激时，人白血病嗜碱性粒细胞产生了慢反应物质（SRS）。[14C] -花生四烯酸（AA）的放射性标记与SRS结合，通过包括二乙氨基乙基纤维素（DEAE -纤维素）、硅酸和反相高效液相色谱（RP - HPLC）在内的几种色谱系统，放射性和生物活性持续共迁移。人嗜碱性白血病SRS表现出与大鼠嗜碱性白血病细胞SRS相似的理化性质。
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