Tang Yijie, Xie Xiyuan, Wu Haixing, Huang Xinlei, Qiu Jiapeng, Han Yupeng, Ke Peng, Zeng Kai, Wu Xiaodan
Department of Anesthesiology, Fuzhou University Affiliated Provincial Hospital, School of Medicine Fuzhou University Fuzhou Fujian China.
Department of Anesthesiology The First Affiliated Hospital of Fujian Medical University Fuzhou China.
Food Sci Nutr. 2025 Sep 26;13(10):e70816. doi: 10.1002/fsn3.70816. eCollection 2025 Oct.
Resveratrol (RES) is a naturally occurring polyphenolic compound possessing neuroprotective properties. This study aims to examine the potential of RES to ameliorate sleep disorder-associated cognitive deficits induced by sleep deprivation (SD) in mice. Adult male C57/BL6 mice were subjected to SD for 72 h, and RES were administrated. Behavioral tests were performed to quantify the cognitive behaviors. RNA-sequencing coupled with bioinformatic analysis was executed to examine gene expression patterns in the hippocampus. Furthermore, the levels of apoptosis and synaptic plasticity were measured by western blotting and histological staining techniques, including the TUNEL and Nissl methods. Morris water maze, novel object recognition and open field tests demonstrated that RES swiftly attenuated the cognitive impairment induced by SD. RNA-sequencing indicated that the mitogen-activated protein kinase (MAPK) pathways were significantly downregulated by RES administration following SD. Additionally, the impaired synaptic plasticity and apoptosis elicited by SD in the hippocampus were improved by RES. SD causes cognitive dysfunction and hippocampal neuronal damage in mice. Treatment with RES improves cognitive function and attenuates neuronal damage in the hippocampus of sleep-deprived mice. Furthermore, the MAPK signaling pathway has been identified as a pivotal target in these neuroprotective effects. These findings will contribute to providing theoretical support for dietary supplementation of RES to prevent or improve cognitive dysfunction caused by sleep problems.
白藜芦醇（RES）是一种具有神经保护特性的天然多酚化合物。本研究旨在探讨RES改善小鼠睡眠剥夺（SD）诱导的睡眠障碍相关认知缺陷的潜力。将成年雄性C57/BL6小鼠进行72小时的SD处理，并给予RES。进行行为测试以量化认知行为。进行RNA测序并结合生物信息学分析以检查海马体中的基因表达模式。此外，通过蛋白质印迹和组织学染色技术（包括TUNEL和尼氏方法）测量细胞凋亡水平和突触可塑性。莫里斯水迷宫、新物体识别和旷场试验表明，RES迅速减轻了SD诱导的认知障碍。RNA测序表明，SD后给予RES可显著下调丝裂原活化蛋白激酶（MAPK）通路。此外，RES改善了SD引起的海马体中受损的突触可塑性和细胞凋亡。SD导致小鼠认知功能障碍和海马神经元损伤。RES治疗可改善睡眠剥夺小鼠海马体的认知功能并减轻神经元损伤。此外，MAPK信号通路已被确定为这些神经保护作用的关键靶点。这些发现将有助于为饮食补充RES以预防或改善由睡眠问题引起的认知功能障碍提供理论支持。
