Li Wei, Ren Ziwen, Wang Tong, Wei Xiaoxia, Wei Hui, Tian Houjun, Xu Ruibin, Dong Jiyang, Wu Liangquan, Huang Biao
Research Institute of Agricultural Quality Standards and Testing Technology, Fujian Academy of Agricultural Sciences, Fujian Key Laboratory of Agro-products Quality & Safety Development & Biotechnology, Fuzhou, Fujian, China.
International Magnesium Institute, College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Front Plant Sci. 2025 Sep 11;16:1667805. doi: 10.3389/fpls.2025.1667805. eCollection 2025.
The issue that the attraction of leaf odors leading to thrips attacks affecting the yield and quality of Passion fruit ()-a high-value tropical crop in southern China has drawn widely attentions. This study aimed to comprehensively investigate the influences of special VOCs in susceptible and resistant passion fruit cultivars on feeding preferences of thrips. To explore the association between VOCs and insect behavior, the aromatic intensity of the selected cultivar leaves was determined. A total of 87 differential volatiles were identified from 423 VOCs using orthogonal partial least squares-discriminant analysis (OPLS-DA, P< 0.05, VIP > 1.0). Metabolic pathway analysis linked the differential volatiles to phenylpropanoid and α-linolenic acid metabolism. The key differential volatiles were fruity odor benzaldehyde and green odor (Z)-3-hexenol, which exhibited the highest rOAVs. Resistant cultivars accumulate high concentrations of benzaldehyde (OAV:191.49), which correlated with thrips attraction; while susceptible cultivars accumulated higher concentration of (Z)-3-Hexenol (OAV: 200.60), associated with repellency. Behavioral assays confirmed thrips preference for benzaldehyde (58% attraction) and aversion to (Z)-3-Hexenol (22% selection). These findings not only could advance our understanding of plant volatile-mediated insect behavior but also enable the development of lures for pest management, while providing a scientific basis for breeding pest-resistant cultivars.
百香果是中国南方一种高价值的热带作物，叶片气味的吸引力会导致蓟马侵袭，进而影响百香果的产量和品质，这一问题已引起广泛关注。本研究旨在全面调查易感和抗性百香果品种中特殊挥发性有机化合物（VOCs）对蓟马取食偏好的影响。为了探究VOCs与昆虫行为之间的关联，测定了所选品种叶片的香气强度。使用正交偏最小二乘法判别分析（OPLS-DA，P<0.05，VIP>1.0）从423种VOCs中鉴定出总共87种差异挥发物。代谢途径分析将这些差异挥发物与苯丙烷类和α-亚麻酸代谢联系起来。关键的差异挥发物是果香味的苯甲醛和青香味的（Z）-3-己烯醇，它们表现出最高的相对气味活性值（rOAVs）。抗性品种积累高浓度的苯甲醛（气味活性值：191.49），这与蓟马的吸引力相关；而易感品种积累较高浓度的（Z）-3-己烯醇（气味活性值：200.60），与驱避性相关。行为试验证实蓟马对苯甲醛有偏好（58%有吸引力），对（Z）-3-己烯醇有厌恶（22%选择）。这些发现不仅可以增进我们对植物挥发性物质介导昆虫行为的理解，还能促进害虫管理诱捕剂的开发，同时为培育抗虫品种提供科学依据。
