血清脂蛋白在琼脂糖凝胶中的显微镜载玻片电泳
Microscope slide electrophoresis of serum lipoproteins in agarose gel.
作者信息
Elphick M C
出版信息
J Clin Pathol. 1971 Feb;24(1):83-5. doi: 10.1136/jcp.24.1.83.
An ultramicro electrophoretic technique for the separation of serum lipoproteins is described using agarose gel on microscope slides.The interaction between the agarose gel and lipoproteins has been reduced and the separation between beta- and pre-beta-lipoproteins has been improved over existing methods. A semiquantitative assay of the lipoproteins can be done in a densitometer. It is suggested that the interaction between agarose gel and lipoproteins, which, without albumin in the system, results in distortion of the lipoprotein bands, may be mediated by free fatty acids.
本文描述了一种在显微镜载玻片上使用琼脂糖凝胶分离血清脂蛋白的超微电泳技术。与现有方法相比，琼脂糖凝胶与脂蛋白之间的相互作用已减少，β-脂蛋白和前β-脂蛋白之间的分离得到了改善。可在密度计中对脂蛋白进行半定量测定。有人认为，在系统中没有白蛋白的情况下，琼脂糖凝胶与脂蛋白之间的相互作用会导致脂蛋白条带变形，这种相互作用可能由游离脂肪酸介导。
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