单纯疱疹病毒在人淋巴细胞中的生长特性
Characterization of the growth of herpes simplex virus in human lymphoid cells.
作者信息
Mizrahi A, Mitchen J R, Von Heyden H W, Minowada J, Moore G E
出版信息
Appl Microbiol. 1972 Jan;23(1):145-54. doi: 10.1128/am.23.1.145-154.1972.
Herpes simplex virus was grown in a 6-liter suspended culture of an atypical permanent human lymphoid cell line, Roswell Park Memorial Institute no. 8226. The kinetics of virus replication were determined by counting viruses by electron microscopy, plaque formation, and tissue culture infectivity. Deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase activity was determined during the course of infection. Electron microscopy studies substantiated the kinetics of the virus infection in lymphoid cells.
单纯疱疹病毒在一种非典型的人类永久性淋巴样细胞系罗斯韦尔帕克纪念研究所8226号的6升悬浮培养物中培养。通过电子显微镜计数病毒、蚀斑形成和组织培养感染性来确定病毒复制的动力学。在感染过程中测定了依赖脱氧核糖核酸的脱氧核糖核酸聚合酶活性。电子显微镜研究证实了淋巴样细胞中病毒感染的动力学。
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