哺乳动物DNA聚合酶中的抗原关系。

Antigenic relationships in mammalian DNA polymerase.

作者信息

Chang L M, Bollum F J

出版信息

Science. 1972 Mar 10;175(4026):1116-7. doi: 10.1126/science.175.4026.1116.

DOI:10.1126/science.175.4026.1116

Abstract

Rabbit antibody was prepared against a high-molecular-weight DNA polymerase purified from the soluble fraction of calf thymus gland. This antibody does not inhibit terminal deoxynucleotidyl transferase isolated from that source, but does inhibit both low-molecular-weight and high-molecular-weight DNA polymerases isolated from cytoplasmic and nuclear fractions of a number of mammalian tissues (mouse L cells, calf thymus, phytohemagglutinin-stimulated human lymphocytes, rat liver, and rabbit bone marrow). The results suggest that (i) no antigenic relationship exists between terminal transferase and DNA polymerase, (ii) common antigenic determinants exist in the DNA polymerases from all mammalian sources, and (iii) multiple forms of DNA polymerase found in mammalian, cells are related by having polypeptide sequences or subunits in common.

摘要

用从小牛胸腺可溶性部分纯化的高分子量DNA聚合酶制备兔抗体。该抗体不抑制从该来源分离的末端脱氧核苷酸转移酶，但抑制从多种哺乳动物组织（小鼠L细胞、小牛胸腺、植物血凝素刺激的人淋巴细胞、大鼠肝脏和兔骨髓）的细胞质和细胞核部分分离的低分子量和高分子量DNA聚合酶。结果表明：（i）末端转移酶和DNA聚合酶之间不存在抗原关系；（ii）所有哺乳动物来源的DNA聚合酶中存在共同的抗原决定簇；（iii）哺乳动物细胞中发现的多种形式的DNA聚合酶通过具有共同的多肽序列或亚基而相关。