Wilson I D
Immunology. 1972 Jun;22(6):1001-11.
Human antibodies which react in a haemagglutination system with secretory IgA, but not serum IgA, were found in serum from 14 per cent of normal subjects, 8 per cent of patients with chronic inflammatory bowel diseases and over 50 per cent of subjects with either peptic ulcer or alcoholic cirrhosis. The specificity of these reactions for secretory IgA was confirmed in inhibition experiments using a panel of immunoglobulin proteins. The anti-secretory piece factors in two sera were found by zone electrophoresis and sucrose density gradient experiments to have physical characteristics most compatible with IgG and IgA proteins, respectively. Further characterization of the specificity of these anti-secretory IgA factors, using a panel of colostral IgA proteins from individual mothers and isolated secretory piece, demonstrated a high degree of variability in antigenic specificity for secretory IgA. In three of five sera tested, the reaction was specific for components in isolated secretory piece. Inhibition studies using products of peptic digestion of colostral IgA suggest that secretory piece is attached to the Fc fragment of the molecule and that digestion releases at least some secretory piece molecules relatively intact. Although the significance of these anti-secretory IgA antibodies is presently unknown, it is anticipated that they will be valuable as reagents in further studies on the molecular structure and biology of secretory IgA.
在14%的正常受试者血清、8%的慢性炎症性肠病患者血清以及超过50%的消化性溃疡或酒精性肝硬化患者血清中，发现了能在血凝系统中与分泌型IgA发生反应，但不与血清IgA发生反应的人抗体。使用一组免疫球蛋白蛋白进行的抑制实验证实了这些反应对分泌型IgA的特异性。通过区带电泳和蔗糖密度梯度实验发现，两份血清中的抗分泌片因子的物理特性分别与IgG和IgA蛋白最为相符。使用来自个体母亲的一组初乳IgA蛋白和分离的分泌片对这些抗分泌型IgA因子的特异性进行进一步表征，结果表明其对分泌型IgA的抗原特异性存在高度变异性。在测试的五份血清中的三份中，该反应对分离的分泌片中的成分具有特异性。使用初乳IgA经胃蛋白酶消化后的产物进行的抑制研究表明，分泌片附着于分子的Fc片段，并且消化释放出至少一些相对完整的分泌片分子。尽管目前尚不清楚这些抗分泌型IgA抗体的意义，但预计它们将作为试剂在进一步研究分泌型IgA的分子结构和生物学方面具有重要价值。
