抗3':5'-环磷酸腺苷二酯酶抗体对盘基网柄菌聚集和分化的抑制作用

Inhibition of aggregation and differentiation of Dictyostelium discoideum by antibodies against adenosine 3':5'-cyclic monophosphate diesterase.

作者信息

Goidl E A, Chassy B M, Love L L, Krichevsky M I

出版信息

Proc Natl Acad Sci U S A. 1972 May;69(5):1128-30. doi: 10.1073/pnas.69.5.1128.

DOI:10.1073/pnas.69.5.1128

PMID:4113864

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC426646/

Abstract

A specific antibody directed against adenosine 3':5'-cyclic monophosphate diesterase (c-AMP diesterase; EC 3.1.4.c), which is secreted by the amoeboid slime mold, Dictyostelium discoideum, was prepared in rabbits. Purified gammaglobulin fractions that contain the antibodies inhibited the activity of cAMP diesterase preparations in vitro and interfered with aggregation and development in vivo. While cells that were treated with antibody were unable to aggregate because of the inability to destroy cAMP, they aggregated normally when washed free of antibody.

摘要

针对由变形虫状黏菌盘基网柄菌分泌的腺苷3':5'-环磷酸二酯酶（c-AMP二酯酶；EC 3.1.4.c）制备了一种特异性抗体，该抗体是在兔体内制备的。含有这些抗体的纯化γ球蛋白组分在体外抑制了cAMP二酯酶制剂的活性，并在体内干扰了聚集和发育。虽然用抗体处理过的细胞由于无法破坏cAMP而无法聚集，但当洗去抗体后它们能正常聚集。