利用二乙酸荧光素和番红精测定培养植物细胞的活力。 - Suppr

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The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.

作者信息

Widholm J M

出版信息

Stain Technol. 1972 Jul;47(4):189-94. doi: 10.3109/10520297209116483.

DOI:10.3109/10520297209116483

PMID:4113995

Abstract

摘要

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The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.

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The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.

作者信息

Widholm J M

出版信息

Stain Technol. 1972 Jul;47(4):189-94. doi: 10.3109/10520297209116483.

DOI:10.3109/10520297209116483

PMID:4113995

Abstract

摘要

相似文献

The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.

Stain Technol. 1972 Jul;47(4):189-94. doi: 10.3109/10520297209116483.

Estimation of viable cell count after fluorescein diacetate staining using phosphorimager analysis.

Biotechniques. 1999 Oct;27(4):685, 688. doi: 10.2144/99274bm12.

The culture of free plant cells and its significance for embryology and morphogenesis.

Curr Top Dev Biol. 1966;1:113-54. doi: 10.1016/s0070-2153(08)60011-3.

[Data on the selective fluorochroming of mast cells].

Acta Histochem. 1970;36(1):74-86.

Live-cell fluorescence imaging to investigate the dynamics of plant cell death during infection by the rice blast fungus Magnaporthe oryzae.

BMC Plant Biol. 2016 Mar 22;16:69. doi: 10.1186/s12870-016-0756-x.

Vacuolar staining methods in plant cells.

Methods Mol Biol. 2015;1242:83-92. doi: 10.1007/978-1-4939-1902-4_8.

Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture.

J Vis Exp. 2017 May 10(123):55442. doi: 10.3791/55442.

A comparison of some methods of preparation of thin sections of virus-infected leaves for electron microscopy.

J Ultrastruct Res. 1967 Aug;19(3):398-407. doi: 10.1016/s0022-5320(67)80227-2.

Quantification of metabolic activity of cultured plant cells by vital staining with fluorescein diacetate.

Anal Biochem. 2013 Oct 1;441(1):58-62. doi: 10.1016/j.ab.2013.06.005. Epub 2013 Jun 27.

Assessment of fluorescein-based fluorescent dyes for tracing Neotyphodium endophytes in planta.

Mycologia. 2013 Jan-Feb;105(1):221-9. doi: 10.3852/12-062. Epub 2012 Jul 16.

引用本文的文献

Huperzine A Production and Acetylcholinesterase Inhibition by Cell Suspension Culture: A Comparative Study in Flasks and an Airlift Bioreactor.

Pharmaceuticals (Basel). 2025 Mar 8;18(3):383. doi: 10.3390/ph18030383.

Induction of somatic embryogenesis and cryopreservation of Boiss.

Front Plant Sci. 2025 Jan 29;16:1535113. doi: 10.3389/fpls.2025.1535113. eCollection 2025.

Gaining or cutting SLAC: the evolution of plant guard cell signalling pathways.

New Phytol. 2024 Dec;244(6):2295-2310. doi: 10.1111/nph.20172. Epub 2024 Oct 6.

Influence of the Culture Conditions on Cell Cultures.

Foods. 2024 Aug 4;13(15):2461. doi: 10.3390/foods13152461.

Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot.

aBIOTECH. 2024 Apr 15;5(2):151-168. doi: 10.1007/s42994-024-00139-7. eCollection 2024 Jun.

Development of a Whole-Cell System Based on the Use of Genetically Modified Protoplasts to Detect Nickel Ions in Food Matrices.

Int J Mol Sci. 2024 May 31;25(11):6090. doi: 10.3390/ijms25116090.

Starch depletion in the xylem and phloem ray parenchyma of grapevine stems under drought.

AoB Plants. 2023 Aug 30;15(5):plad062. doi: 10.1093/aobpla/plad062. eCollection 2023 Oct.

Early selection of carrot somatic hybrids: a promising tool for species with high regenerative ability.

Plant Methods. 2023 Oct 7;19(1):104. doi: 10.1186/s13007-023-01080-4.

Fluorescein staining of chloroplast starch granules in living plants.

Plant Physiol. 2024 Jan 31;194(2):662-672. doi: 10.1093/plphys/kiad528.

Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage ( var. L.) Cultivars.

Plants (Basel). 2023 Aug 27;12(17):3074. doi: 10.3390/plants12173074.