Mikulska T, Mikulski P, Rogulski J
J Clin Pathol. 1972 Oct;25(10):856-62. doi: 10.1136/jcp.25.10.856.
A new family with an electrophoretically more slowly moving albumin variant is described. The pattern of variance was studied using three different electrophoretic techniques, and compared with certain other known fast and slow variants. Features of penicillin and bromphenol blue binding by normal and variant albumins were also studied. Both normal human albumin and the albumin variant treated with penicillin broadened and moved faster. Native albumin A, prestained with bromphenol blue, binds approximately twice as much of the dye as the slow-moving variant. On the other hand, staining of denatured proteins on paper showed that both bands take up equal amounts of dye.
本文描述了一个新的家族，其白蛋白变体在电泳时迁移速度较慢。使用三种不同的电泳技术研究了变异模式，并与其他一些已知的快速和慢速变体进行了比较。还研究了正常和变异白蛋白与青霉素和溴酚蓝结合的特性。正常人白蛋白和用青霉素处理的白蛋白变体均变宽且迁移速度加快。用溴酚蓝预染的天然白蛋白A结合的染料量约为慢速迁移变体的两倍。另一方面，纸上变性蛋白质的染色显示两条带吸收的染料量相等。
